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pDEST-Spe3-RfA-HA

Plasmid

#186374

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-HA-RfA

Plasmid

#186373

Purpose

Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-Venus-RfA

Plasmid

#186371

Purpose

Destination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Chimera

Plasmid

#188802

Purpose

Chimeric Avs4 with transmembrane effector

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4 chimera

Expression

Bacterial

Promoter

EcAvs4 native promoter

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Kan

Plasmid

#188928

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Kan

Expression

Yeast

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-DHFR-3xHA

Plasmid

#188934

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-DHFR-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Avs4_Archaeon_isolate_HyVt-335

Plasmid

#188800

Purpose

Avs4 from Archaeon isolate HyVt-335

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Avs4

Expression

Bacterial

Promoter

lac

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA018

Plasmid

#185753

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaTip1;1 (promoter)

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6730

Plasmid

#139155

Purpose

PapFAB45 - TARGET3 - MevT - MBIS

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

MevT-MBIS

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6731

Plasmid

#139156

Purpose

Pstabilized - TARGET8 - MevT - MBIS

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

MevT-MBIS

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6721

Plasmid

#139152

Purpose

PDNsp1 - TARGET8 - sfGFP

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6722

Plasmid

#139153

Purpose

Pstabilized - TARGET8 - sfGFP

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6729

Plasmid

#139154

Purpose

PgadE - TARGET3 - MevT - MBIS

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

MevT-MBIS

Expression

Bacterial

Mutation

N/A

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge