We narrowed to 18,489 results for: Met

1. pre-Ubc4 BrG pUC19

   Plasmid

   #111322

   Purpose

   for transcription to Ubc4 pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

   Insert

   UBC4 (UBC4 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   intron has branch-site A to G mutation

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. rpaA_NS1

   Plasmid

   #101829

   Purpose

   drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA with its native promoter
   

   Use

   Cyanobacteria cloning vector

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. P0050::rpaA

   Plasmid

   #101830

   Purpose

   drives expression of rpaA from an rpaA-independent promoter

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   RpaA with p0050 promoter (+ gentamicin resistance casette)
   

   Use

   Cloning vector

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ptrc::galP_NS1

   Plasmid

   #101843

   Purpose

   drives expression of galP transporter from NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   Ptrc promoter driving GalP transporter expression
   

   Use

   Cloning vector

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

   Plasmid

   #90036

   Purpose

   TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

   Depositor

   Matthew Gibson

   Article

   Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

   Insert

   7R_Ex2-LEFT
   

   Use

   TALEN

   Tags

   3x Flag and Fok1-DD domain

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

   Plasmid

   #90037

   Purpose

   TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

   Depositor

   Matthew Gibson

   Article

   Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

   Insert

   7R_Ex2-RIGHT
   

   Use

   TALEN

   Tags

   Fok1-RR domain and HA

   Promoter

   CMV

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPMS008 (JBEI-11596)

   Plasmid

   #87957

   Purpose

   jStack yeast assembly into plant vectors, Level 1 Intermediary Vector, ColE1origin

   Depositor

   Dominique Loque

   Article

   Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSALECT-SupH

   Plasmid

   #59710

   Purpose

   pSALECT with SupH insert

   Depositor

   Christopher Anderson

   Article

   Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

   Insert

   SupH
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSALECT-AhyI

   Plasmid

   #59708

   Purpose

   pSALECT with AhyI insert

   Depositor

   Christopher Anderson

   Article

   Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

   Insert

   AhyI
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSABAD14-750

    Plasmid

    #45616

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    MjaTFIIBC53
    

    Tags

    GB1-H6

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-eIF4E-S200C

    Plasmid

    #37229

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    eIF4E
    

    Expression

    Bacterial

    Mutation

    S200C

    Promoter

    T7

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pTYB2-eIF4B-A130C

    Plasmid

    #37227

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF3
    

    Expression

    Bacterial

    Mutation

    A130C

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pUC19-DAD4

    Plasmid

    #37224

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    DAD4 (DAD4 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSBBi-RP CCS1(Δ304-316)

    Plasmid

    #139163

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    caffeine synthase 1 [Coffea arabica] CCS-CTS region deletion
    

    Use

    Transposon

    Expression

    Mammalian

    Mutation

    deleted amino acids 304-319

    Promoter

    EF1α

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mNG-IRES-mScarlet-dMCP

    Plasmid

    #231089

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    mNG-IRES-mScarlet-dMCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mNG-IRES-mScarlet-dPCP

    Plasmid

    #231090

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dPCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    mNG-IRES-mScarlet-dPCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-6xHis-MBP-TEV site-TadA8.20

    Plasmid

    #194702

    Purpose

    Expresses N-terminal MBP fused TadA8.20 in bacteria

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    TadA8.20
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    T7 promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIR-FbALFA

    Plasmid

    #184678

    Purpose

    NIR-Fb to ALFA-tag

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-FbALFA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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