We narrowed to 15,923 results for: nans

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AAVS-HsProm1s1-StayGold

Plasmid

#211333

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196988

Purpose

Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

Depositor

Elizabeth Kirby

Article

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Nestin-dCas9-KRAB-T2a-GFP

Use

CRISPR and Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEK22

Plasmid

#210588

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH3pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdH3pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-OptoPB

Plasmid

#113935

Purpose

Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

OptoPB

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

Plasmid

#114106

Purpose

fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

Insert

Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 SENP6

Plasmid

#14583

Depositor

Mary Dasso

Article

Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

Insert

SENP6 (SENP6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAB078d8

Plasmid

#79206

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-B-pGAS5

Plasmid

#46371

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.

Insert

GAS5 (GAS5 Human)

Use

Luciferase

Tags

luciferase

Mutation

promoter

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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MKK2

Plasmid

#29580

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK2 (MAP2K2 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively active mutant; Starting Met remove…

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pHRSin-IRES2-EmGFP_HPC-4 Ab light

Plasmid

#187359

Purpose

Lentiviral expression of HPC-4 antibody light chain

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody light chain

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-fibcon-iCAM1

Plasmid

#205212

Purpose

This vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG

Plasmid

#127300

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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