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TEG +H122D

Plasmid

#187434

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 128 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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FER +H111E

Plasmid

#187429

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 117 to glutamic acid

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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KEE +E111H

Plasmid

#187431

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed glutamic acid at 117 to histidine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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SNG +R111Q

Plasmid

#187437

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed arginine at 117 to glutamine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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FER +H122D

Plasmid

#187428

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 128 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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MON +N122H

Plasmid

#187433

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed asparagine at 128 to histidine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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OST +Q111R

Plasmid

#187436

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed glutamine at 116 to arginine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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FER +H111T

Plasmid

#187430

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 117 to threonine

Promoter

PH and p10

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CHI +N122D

Plasmid

#187427

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed asparagine at 130 to aspartic acid

Promoter

PH and p10

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCBH-Cas9-Suntag-BB_BbsI

Plasmid

#164805

Purpose

REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pUASp-Flag-Myc-Qin-Tudor-attB

Plasmid

#44210

Depositor

Phillip Zamore

Article

Zhang et al Mol Cell. 2011 Nov 18;44(4):572-84. doi:

Insert

Qin-Tudor-domain (qin Fly)

Use

Fly transformation

Tags

Flag and Myc

Mutation

lacks E3 domain on N-term (del aa1-274)

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a D540K

Plasmid

#53057

Purpose

Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Aspartate 540 to Lysine

Promoter

SP6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv4 S100D

Plasmid

#49094

Purpose

expression of Etv4 S100D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 S100D (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Mutation

S100D

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L113E

Plasmid

#47339

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L113E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock F122D

Plasmid

#47340

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

F122D

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/BmalF423R

Plasmid

#47348

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

F423R

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

Plasmid

#209081

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T, NAC deletion (aa60-95)

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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