We narrowed to 36,257 results for: NAM

1. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

   Plasmid

   #127407

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon, K305H,…

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

   Plasmid

   #127401

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon, W340A,…

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS-ATG1-ATG7 3'UTR(406)

   Plasmid

   #122070

   Purpose

   Expressing ATG1 with ATG7 3'UTR

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   The native 3'UTR was replaced by ATG7 3'…

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-myr-Citrine-SV40pA-FKF

   Plasmid

   #119865

   Purpose

   Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   2x myristoylation

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-MTX-u36_Snifit

   Plasmid

   #119045

   Purpose

   Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)

   Depositor

   Kai Johnsson

   Article

   Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

   Insert

   MTX-u36_Snifit
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   36TAG R44L H45Q

   Promoter

   pBAD

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-MTX-u51_Snifit

   Plasmid

   #119046

   Purpose

   Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)

   Depositor

   Kai Johnsson

   Article

   Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

   Insert

   MTX-u51_Snifit
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   51TAG R44L H45Q

   Promoter

   pBAD

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-MTX-u23_Snifit

   Plasmid

   #119044

   Purpose

   Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)

   Depositor

   Kai Johnsson

   Article

   Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

   Insert

   MTX-u23_Snifit
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   23TAG R44L H45Q

   Promoter

   pBAD

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHES 946

   Plasmid

   #112644

   Purpose

   blue light inducible cryptochrome based optogenetic expression system

   Depositor

   Hana El-Samad

   Article

   Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

   Insert

   RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Synthetic, Budding Yeast, Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAD-GH-Mefv iso1

   Plasmid

   #92107

   Purpose

   Yeast two hybrid

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-SERTR596Q-myc

    Plasmid

    #107483

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y289I

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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