We narrowed to 10,853 results for: ENA

1. EGFP-Cyclin B1

   Plasmid

   #215049

   Purpose

   Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

   Plasmid

   #216248

   Purpose

   HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human RANGAP1 (RANGAP1 Human)
   

   Use

   CRISPR and TALEN; Hdr template

   Tags

   miniIAA7.3xFlag.P2A.BSD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEVOL-pAzF[TAG]

   Plasmid

   #164579

   Purpose

   Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mj pCNFRS[TAG] (aaRS1)
   Mj pCNFRS[TAG] (aaRS2)
   Mj tRNA[TAG]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   araBAD, glnS, and proK

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSPL3-ABCC8-2

   Plasmid

   #135916

   Purpose

   Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT259 Lenti pEF-dCas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast

   Plasmid

   #187335

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Cas9-3XFLAG-VP64-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. kiCAP-AAV-MyoAAV1C

   Plasmid

   #224438

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 1C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   RGDLSTP insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-APEX2

   Plasmid

   #215127

   Purpose

   Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   APEX2 (APEX2 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3. Flag-CSL R192H

   Plasmid

   #167005

   Purpose

   Mammalian expression of CSL R192H

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R192H

   Promoter

   CMV

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SspB-HaloTag-ITSN1-DHPH

   Plasmid

   #176128

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VGlut1 scFv [N28/9]

    Plasmid

    #182084

    Purpose

    Mammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-V3-CD20-Puro

    Plasmid

    #209757

    Purpose

    Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-tetO-loxP-hKLF2

    Plasmid

    #60850

    Purpose

    Lentiviral DOX-inducible expression vector for human KLF2

    Depositor

    Rudolf Jaenisch

    Article

    Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. SHIP2-WT

    Plasmid

    #214907

    Purpose

    expression of the full length variants of the SHIP2

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe.puro.CDK8.KD.flag

    Plasmid

    #19759

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D173A (kinase dead)

    Promoter

    Available since

    Dec. 11, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid

    #208956

    Purpose

    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSPL3-ABCC8-9-10

    Plasmid

    #135918

    Purpose

    Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

    Plasmid

    #208946

    Purpose

    A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. kiCAP-AAV-MyoAAV2E

    Plasmid

    #224441

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 2E, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDQGYQ insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. kiCAP-AAV-MyoAAV3D

    Plasmid

    #224445

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDYREL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. kiCAP-AAV-MyoAAV3E

    Plasmid

    #224446

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3E, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDHGVL insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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