We narrowed to 11,284 results for: VARS

1. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

   Plasmid

   #223323

   Purpose

   Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Insert

   SpCas9 and sgRNA-T2 (cas9 )
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

   Plasmid

   #220609

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG8Ha-EWS_RRM(359-447)

   Plasmid

   #188045

   Purpose

   Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 446-656

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG8Ha-EWS_RRM-RGG(359-513)

   Plasmid

   #188046

   Purpose

   Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His8

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-358, 514-656

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEmal10H-MBP-EWSR1-449-513

   Plasmid

   #199439

   Purpose

   Expresses RG2 (the second RG-rich region, 449-513) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-448, 514-656

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. macroH2A2-GFP (pc2191)

   Plasmid

   #223709

   Purpose

   Expression of macroH2A2 tagged N-terminal to GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Control-TP53-LRG-GFP

   Plasmid

   #225874

   Purpose

   Guides targeting P53 and a control sequence

   Depositor

   Janai Carr-Ascher

   Article

   Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

   Insert

   Control and TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Control-RB1-LRG-GFP

    Plasmid

    #225875

    Purpose

    Guides targeting RB1 and a control sequence

    Depositor

    Janai Carr-Ascher

    Article

    Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

    Insert

    Control and RB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain

    Plasmid

    #187277

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    deltaAnillin homology domain

    Promoter

    U6, CMV

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458-3xHA-dFnCas9

    Plasmid

    #201954

    Purpose

    Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dFnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A and H969A on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-dSpCas9

    Plasmid

    #201953

    Purpose

    Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-dSpCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A and H840A on SpCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE FL NAP1 STOP C' EGFP IRES Blast

    Plasmid

    #179102

    Purpose

    Expresses untagged full length NAP1

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-N'-FLAG-HA-NAP1S318A

    Plasmid

    #195410

    Purpose

    Expresses FLAG-HA tagged human NAP1 with S318A point mutation

    Depositor

    Alicia Pickrell

    Article

    Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

    Insert

    Nak associated protein 1 (AZI2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Serine 318 turned to Alanine

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ERAP1-rs27895(T)

    Plasmid

    #206143

    Purpose

    Expresses the rs27895-T allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Tags

    myc

    Mutation

    G346 changed to D346

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2/shCol1a1/Scarlet_Seq1.3

    Plasmid

    #206137

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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