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pDONR223_SERPINB5_p.D141A

Plasmid

#82735

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. D141A; p. 176S>P; p.190_192delTDTins25amino…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A7T

Plasmid

#82736

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. A7T; p.176S>P; p. 190_192delTDTins25; p.194…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A42F

Plasmid

#82738

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p.A42F; p.176S>P; p.190_192delTDTins25; p.194_…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A165T

Plasmid

#82741

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. A165T; p.176S>P; p.190_192delTDTins25; p.19…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAVCR2_p.P6S

Plasmid

#82742

Purpose

Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

HAVCR2 (HAVCR2 Human)

Use

Gateway entry vector

Mutation

p.P6S; p.R140L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

Plasmid

#96888

Purpose

expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36, mutated lysine 351 to ar…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3b

Plasmid

#180433

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3c

Plasmid

#180434

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3d

Plasmid

#180435

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_omicron_BA.1

Plasmid

#180375

Purpose

Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

Spike (omicron BA.1 variant) (S SARS-CoV-2)

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

c terminal D18

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.ELREA_746del

Plasmid

#82911

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Deletion of residues 746-750 (ELREA)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (CH.1.1)

Plasmid

#241112

Purpose

Expresses SARS-CoV-2 spike (CH.1.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (CH.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147E,W152R,…

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYX233-b2delta-DHFR

Plasmid

#163760

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2delta-DHFR (Dhfr Budding Yeast, Mouse)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.769_770insASV

Plasmid

#82880

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

ASV insertion between AA 769 and 770

Promoter

None

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.774_775insHV

Plasmid

#82852

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

HV insertion between AA 774 and 775

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.773_774insH

Plasmid

#82855

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

Additional H residue inserted between AA 773 and …

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

Plasmid

#197502

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

Plasmid

#197503

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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dRED

Plasmid

#139836

Purpose

Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

RNase H1 protein contains D210N mutation. dCas9 …

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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