We narrowed to 15,731 results for: LEA

1. pRSFDuet-1_VNp-mNeongreen-Erythropoietin

   Plasmid

   #182423

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-eythropoietin (EPO Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB N-term-mTurquoise2

   Plasmid

   #169222

   Purpose

   Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

   Plasmid

   #187579

   Purpose

   Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PSPC1 (PSPC1 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK6-HA-TAK1

   Plasmid

   #44160

   Depositor

   Xin Lin

   Article

   Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

   Insert

   Tak1 (Map3k7 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

6. EGFP-HsNRF2 (NFE2L2)

   Plasmid

   #194303

   Purpose

   Mammalian expression of human NRF2 (NFE2L2) fused to EGFP at the N-terminus. Parton lab clone KTH

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   NFE2L2 (NFE2L2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-PyronicSF/pCMV-myc-nuc

   Plasmid

   #124814

   Purpose

   Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Insert

   PyronicSF
   

   Tags

   Nuclear Localization Signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

   Plasmid

   #187574

   Purpose

   Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   RNPS1 (RNPS1 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

   Plasmid

   #187585

   Purpose

   Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NPAT (NPAT Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ shSTING-Mmu

    Plasmid

    #127697

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse STING gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shSTING Mmu (Sting1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 7TFP CDH1 mutant reporter

    Plasmid

    #91703

    Purpose

    lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    CDH1 mutant promoter (CDH1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    mutant E-boxes

    Promoter

    CDH1 mutant

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

    Plasmid

    #235091

    Purpose

    SRSF3 mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. TVBB C-term-mScarlet

    Plasmid

    #169219

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. B-Sniper SpCas9

    Plasmid

    #207361

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCWXPG-UBI-Ngn3PDX1MafA

    Plasmid

    #114303

    Purpose

    Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    Ngn3 (NEUROG3 Human)
    

    Use

    Lentiviral

    Mutation

    Ngn3, PDX1 and MafA proteins are expressed from a…

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

    Plasmid

    #187584

    Purpose

    Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SP100 (SP100 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDsRed-importinα

    Plasmid

    #119679

    Purpose

    Expresses DsRed-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    importinα (KPNA2 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Contains amino acids 251-529

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-actin-R62D

    Plasmid

    #118379

    Purpose

    Gateway compatible donor vector with actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    R62D

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HS-GFP-PACT

    Plasmid

    #105954

    Purpose

    heat shock inducible transgenesis, PACT is a centriol marker

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PACT (AKAP9 Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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