We narrowed to 15,601 results for: LEA

1. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ID1-pLVXT

   Plasmid

   #119280

   Purpose

   Doxycycline-inducible mouse ID1 from 806 cells

   Depositor

   Joan Massague

   Article

   Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB N-term-mScarlet

   Plasmid

   #169218

   Purpose

   Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDipA-TEV-His12

   Plasmid

   #137042

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-BLM DM

   Plasmid

   #80071

   Purpose

   Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

   Depositor

   Nathan Ellis

   Article

   Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

   Insert

   BLM (BLM Human)
   

   Use

   Tags

   GFP-BLM

   Expression

   Mammalian

   Mutation

   K317R (AAA>AGA) and K331R (AAA>AGA)

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pELPS-eDHFR-YFP-T2A-Renilla

   Plasmid

   #193253

   Purpose

   Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

   Depositor

   Mark Sellmyer

   Article

   Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

   Insert

   The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
   

   Use

   Lentiviral

   Tags

   Renilla luciferase (rLuc) and yellow fluorescent …

   Expression

   Mutation

   None

   Promoter

   EF1a

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

   Plasmid

   #83107

   Purpose

   3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

   Depositor

   Andrew Brooks

   Inserts

   c-Myc (MYC Human)
   CDK4 (CDK4 Human)
   eGFP
   

   Use

   Lentiviral

   Tags

   2A self cleaving peptide

   Expression

   Mammalian

   Mutation

   R24C and p64 isoform

   Promoter

   hPGK and hPGK (single transcript)

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2-TetR-neoR

   Plasmid

   #153310

   Purpose

   Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Insert

   TetR
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMVA2

   Plasmid

   #119951

   Purpose

   Expresses recombinant MVA pathway in Escherichia coli

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   mvaE
   mvaS
   mvaK1
   mvaK2
   mvaD
   idi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A110G

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ230-RR

    Plasmid

    #108860

    Purpose

    LbCpf1 Gateway entry plasmid with G548R and K611R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R and K611R, Cpf1 is rice codon optimized

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-HP1α-miRFP670-Cry2WT

    Plasmid

    #122442

    Purpose

    Expresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Use

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-TEV-U1AF37MF77M

    Plasmid

    #168267

    Purpose

    Expresses human dmU1A with the F37M/F77M double mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    U1A RRM1 crystallization module (SNRPA Human)
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiU6-LacZ-GFP-Puro (BB)

    Plasmid

    #170459

    Purpose

    For cloning gRNA block

    Depositor

    Yonglun Luo

    Article

    Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

    Insert

    hU6-BB-LacZ and EF1A-EGFP-2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 for gRNA and EF1A for EGFP-2A-Puro

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUW-TetO-Tead4

    Plasmid

    #128832

    Purpose

    dox-dependent expression of mouse Tead4

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Tead4 (Tead4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. OCT4 GFP puro Donor 1

    Plasmid

    #22209

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

    Plasmid

    #133044

    Purpose

    Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

    Insert

    Xph20-ETWV
    

    Use

    Tags

    NLS, TEV protease + TEV cleavage site, and miRFP6…

    Expression

    Mammalian

    Mutation

    S63K

    Promoter

    CAG

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. TVBB C-term-mTurquoise2

    Plasmid

    #169223

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

    Plasmid

    #163572

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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