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AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Publication

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Publication

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Publication

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-YAP5SA

Plasmid

#223189

Purpose

Plasmid containing the Zfr/YAP5SA cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Publication

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

YAP5SA (Yap1 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-P2A-mCherry-pA

Plasmid

#233027

Purpose

To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d/mCherry

Use

AAV

Tags

mCherry

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-P2A-mCherry-pA

Plasmid

#233026

Purpose

To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site

Depositor

Jonathan Ploski

Publication

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d/mCherry

Use

AAV

Tags

mCherry

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

Plasmid

#220597

Purpose

Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

mVenus-Q69M (ME)

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

EF1a

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_g5-HT2h

Plasmid

#208715

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Publication

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201820

Purpose

An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

Depositor

Christopher Richie

Publication

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AiP15143 - pAAV-AiE0452h-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN5143)

Plasmid

#224193

Purpose

AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

mScarlet-10aa-H2B

Use

AAV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15050 - pAAV-AiE1426m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5050)

Plasmid

#224192

Purpose

AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15041 - pAAV-AiE1417m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5041)

Plasmid

#224187

Purpose

AiE1417m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EBFP_Donor

Plasmid

#178089

Purpose

HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Publication

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBFP (T65S/F145Y)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Publication

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1 sgRNA

Promoter

U6

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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TRE-dCas9(sa)-VP64-AAV

Plasmid

#213035

Purpose

dCas9-VP64 expressed under control of TRE

Depositor

Nadav Ahituv

Publication

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

dCas9

Use

AAV and CRISPR

Tags

NLS and NLS-VP64

Promoter

TRE, miniCMV

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-2pabPAC

Plasmid

#234547

Purpose

to express the optogenetic tool 2pabPAC, which increases cAMP levels when stimulated by blue light, in astrocytes

Depositor

Hajime Hirase

Publication

Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

2pabPAC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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DRH016_ AAV-hSyn-DN-THR

Plasmid

#225089

Purpose

Expression of dominant-negative thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

Depositor

Bernardo Sabatini

Publication

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CRF0.5

Plasmid

#208660

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner

Depositor

Yulong Li

Publication

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-EGFP-Sensor (tdT)-tTA2-WPRE

Plasmid

#259669

Purpose

Detecting tdTomato mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct.

Depositor

Jerzy Szablowski

Publication

Watanabe et al Nat Commun. 2026 May 27. doi: 10.1038/s4

Insert

RNA sensor for tdTomato

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

Nov. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-EGFP-Sensor (cfos)-tTA2-WPRE

Plasmid

#259671

Purpose

Detecting mouse Fos mRNA and induces tTA2 expression under hSyn1 promoter. tTA2 subsequently activates reporter gene expression from a TRE-driven construct.

Depositor

Jerzy Szablowski

Publication

Watanabe et al Nat Commun. 2026 May 27. doi: 10.1038/s4

Insert

RNA sensor for cfos

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

Nov. 2, 2026

Availability

Academic Institutions and Nonprofits only

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