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pAAV2/1-CAG-Flex-FlpO-WPRE

Plasmid

#171690

Purpose

Cre-dependent expression of Flp-recombinase

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

Flip-recombinase

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U-TVA950

Plasmid

#115236

Purpose

Lentiviral transfer plasmid for expression of TVA950

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TVA950

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 AlcR

Plasmid

#153410

Purpose

Ethanol inducible system trans activator

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

AlcR CDS

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS5x

Plasmid

#155113

Purpose

5' entry vector containing QUAS5x promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x

Use

Gateway 5' entry vector

Promoter

QUAS5x-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Promoter

QUASM1-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL30009

Plasmid

#50301

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal Myc tag (4x Myc)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Promoter

UAS

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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HcRed-pcw107-V5

Plasmid

#64647

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Promoter

PGK

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

Plasmid

#141198

Purpose

Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids and amino acids 6…

Promoter

CMV

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCOTS-pyl-GFP(35TAG)

Plasmid

#92047

Purpose

This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

Depositor

Lital Alfonta

Article

Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

Inserts

EGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)

Use

Replicative expression plasmid for cyanobacteria …

Tags

Histag

Mutation

Tyrosine in position 35 of the GFP was mutated f…

Promoter

LeuP (native S. elongatus promoter), PpsbII, and …

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pGWB443

Plasmid

#74829

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PLEXIN A1

Plasmid

#58237

Purpose

Riboprobe for in vitro transcription of Plexin A1

Depositor

Christiana Ruhrberg

Article

Bouvree et al Circ Res. 2012 Aug 3;111(4):437-45. doi:

Insert

Plexin A1 (Plxna1 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 FL (1-440)

Plasmid

#210425

Purpose

Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

Depositor

Carsten Sachse

Article

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph20_eGFP_CCR5TC

Plasmid

#187444

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Right-G1397-N

Plasmid

#169213

Purpose

Express DdCBE for ND5 Point Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Expression

Mammalian

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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p3s-ND5-Left-G1397-C

Plasmid

#169212

Purpose

Express DdCBE for ND5 Point Mutation

Depositor

Jin-Soo Kim

Article

Lee et al Nat Commun. 2021 Feb 19;12(1):1190. doi:

Insert

DdCBE for ND5 mutation

Expression

Mammalian

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–dSpCas9

Plasmid

#157834

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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