We narrowed to 150,533 results for: ins
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Plasmid#79731PurposeThis plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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AAV2_hSyn_iChloC_CA_Citrine
Plasmid#98220PurposeSlow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChloC_C128A gene
UseAAVTagsCitrineExpressionMammalianMutationE83Q, E90R, E101S, C128A, T159C, D156NPromoterhuman synapsinAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
TagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a C2AB
Plasmid#40051DepositorInserthSlp2-a C2AB (SYTL2 Human)
TagsEGFPExpressionMammalianMutationC2AB domains only (aa 597-910)PromoterCMV promoterAvailable SinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
AT1G66830 X005_pECIA14
Plasmid#114810PurposePrey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.DepositorInsertAT1G66830 (AT1G66830 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0
Plasmid#165984PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.DepositorInsertsRK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGE1.0
Plasmid#165986PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
spectinomycin/streptomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…Available SinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIE-C-hα2M-H6
Plasmid#128494PurposeExpresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (R787-N1473) (A2M Human)
TagsHis tag (6x)ExpressionInsectMutationcontains R787-N1473PromoterIE1Available SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK16_HUMAN_D0
Plasmid#79696PurposeThis plasmid encodes the kinase domain of CDK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Blnk-SH2
Plasmid#46408DepositorInsertBlnk (BLNK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 333-456PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mTagBFP
Plasmid#105789PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mTagBFPExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap2-SH2
Plasmid#46435DepositorInsertGrap2 (GRAP2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 52-159PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Fgr-SH2
Plasmid#46427DepositorInsertFgr (FGR Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 137-245PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
AT5G48740 O3_pECIA2
Plasmid#114963PurposeBait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.DepositorInsertAT5G48740 (AT5G48740 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT5G59650 O4_pECIA2
Plasmid#114964PurposeBait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.DepositorInsertAT5G59650 (AT5G59650 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
DAPK1_HUMAN_D0
Plasmid#79728PurposeThis plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)
Plasmid#169166PurposeBaculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cellsDepositorInsert3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
Tags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)
Plasmid#171102PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.DepositorAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (NLS-P426)
Plasmid#171538PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
ExpressionMammalianMutationan SV40 NLS sequence (PKKKRKVGI) insertion into t…Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only