We narrowed to 36,257 results for: NAM

1. pClneoMyc'-EGL-44

   Plasmid

   #66855

   Purpose

   Express Myc-tagged C.elegans EGL-44 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

   Insert

   EGL-44 (CELE_F28B12.2 Nematode)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTW094

   Plasmid

   #64757

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-6xHIS epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT005

   Plasmid

   #64760

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 2xT7 to an HA-6xHIS epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW4.28

   Plasmid

   #62878

   Purpose

   Bacterial expression of MBP-MEG-3 T541A S582A T605A

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MEG-3 T541A S582A T605A
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   T541A S582A T605A

   Promoter

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNB786

   Plasmid

   #60151

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNB682

   Plasmid

   #60143

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   A4V

   Promoter

   MET17pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW3.31

   Plasmid

   #62865

   Purpose

   Yeast two-hybrid bridge vector expressing PAA-1

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   PAA-1/Y3H
   

   Use

   Two-hybrid bridge vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNB693

   Plasmid

   #60145

   Purpose

   Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)

   Plasmid

   #52898

   Purpose

   YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Ypd*1-L-IFP-F[2]
   

   Use

   Tags

   L-IFP-F[2]

   Expression

   Yeast

   Mutation

   Changed Tryptophane 80 to Alanine

   Promoter

   GAL1

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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