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NODALvar-matMYC

Plasmid

#115249

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-T7TS

Plasmid

#115251

Purpose

For in vitro transcription of a human NODAL splice variant open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG31TAG

Plasmid

#188996

Purpose

Express chimadanin with TAG at 28 and 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 and 31position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi31TAG

Plasmid

#188985

Purpose

Express chimadanin with TAG at 31 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 31 position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4_KBM

Plasmid

#187800

Purpose

DUX4 with KBM (p. E414-L424 deletion) with MAC-C tag

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4-KBM (DUX4 Human)

Expression

Mammalian

Mutation

p. E414-L424 deletion

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_NTRK3

Plasmid

#187796

Purpose

MAC-tagged gene expression of human NTRK3

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

NTRK3 (NTRK3 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_RET

Plasmid

#187775

Purpose

MAC-tagged gene expression of human RET

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RET (RET Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-FERM

Plasmid

#187371

Purpose

Expresses human Plekhh1-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-CTer

Plasmid

#187369

Purpose

Expresses human Plekhh1-CTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-CTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-NTer

Plasmid

#187368

Purpose

Expresses human Plekhh1-NTer labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-NTer

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMA5136

Plasmid

#184853

Purpose

A retroviral vector encoding hygromycin resistance and PhiC31 recombinase

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

PhiC31o recombinase

Use

Retroviral; Phic31 attp-attb recombination/excisi…

Expression

Mammalian

Mutation

Codon-optimized for E. coli expression

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4659

Plasmid

#184854

Purpose

A retroviral vector with G418 resistance for cloning inserts between PhiC31 attP and attB sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Retroviral; Phic31 attp-attb recombination/excisi…

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S(-31)-M30_dT7term

Plasmid

#112253

Purpose

Expresses the S(-31)-M30 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S*5-M5_dT7term

Plasmid

#112254

Purpose

Expresses the S*5-M5 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S*5-M5 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-EnR

Plasmid

#185497

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Yan et al Dev Biol. 2009 May 1;329(1):80-95. doi:

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

fusion of winged helix domain with EnR domain

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRK011

Plasmid

#69875

Purpose

expression of Channelrhodopsin-2 T159C mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

T159C

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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SITS-ccdb-mCherry

Plasmid

#118753

Purpose

Multisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFF

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Tags

mCherry

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV-mCerulean-tDeg

Plasmid

#185401

Purpose

mCerulean-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

mCerulean-tDeg

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-tiger-Caspase-1/4b

Plasmid

#183372

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T-Y10AL15A_D

Plasmid

#146153

Purpose

Insect Expression of Dm4E-T-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-Y10AL15A (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_D

Plasmid

#146150

Purpose

Insect Expression of Dm4E-T

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T (4E-T Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4725

Plasmid

#178796

Purpose

Pdpy-30 DAF-2 unc-54 3' UTR C.elegans pan-tissue expression of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pdpy-30

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH3113

Plasmid

#178795

Purpose

Pgref-1KPC-1 mini-gene unc-54 3' UTR C.elegans neuron and tissue expression of KPC-1

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

KPC-1 (kpc-1 Nematode)

Expression

Worm

Promoter

Prgef-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH1498

Plasmid

#178600

Purpose

Prgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-18 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-18 (ins-18 Nematode)

Expression

Worm

Promoter

Prgef-1

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Lmajor_gp63_10_0460_D463N

Plasmid

#171644

Purpose

Expression of L. major glcyoprotein-63 (D463N) with substrate binding site mutation from chromosome 10 in mammalian cells

Depositor

Dennis Ko

Article

Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e

Insert

LmjF.10.0460 D463N

Tags

Myc-His

Expression

Mammalian

Mutation

D463N

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCm No carrier NikJC199U

Plasmid

#174361

Purpose

Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

Depositor

Brandon Greene

Article

Caceres et al Methods Enzymol. 2022;662:119-141. doi:

Insert

nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]

Tags

8xHis and TEV

Expression

Bacterial

Mutation

C199U

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS181

Plasmid

#177637

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

HaloTag

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS212

Plasmid

#177639

Purpose

In vitro transcription of Patiria miniata Lamp1

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Lamp1

Use

In vitro transcription

Tags

mCherry

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pZS217

Plasmid

#177640

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS221

Plasmid

#177643

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K57A, K58A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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