We narrowed to 63,660 results for: cell

1. pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

   Plasmid

   #209056

   Purpose

   Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 deltaexon-8
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-858_CD19-BBz_CAR_TFAP4

   Plasmid

   #207503

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4, CD19-BBz_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO5-SgHottip-GFP-CRISPRi

   Plasmid

   #134989

   Purpose

   dCas9-mediated inactivation of HOTTIP in mammalian cells

   Depositor

   Suming Huang

   Article

   Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

   Insert

   HOTTIP (HOTTIP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 330-cherry-hCYP3A4-enhancer-L-sgRNA

   Plasmid

   #176840

   Purpose

   Targeting human CYP3A4 proximal enhancer left boundary. sgRNA expressing cells could be FACS sorted by cherry expression.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer left boundary
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-RSLID1(WT)

   Plasmid

   #122296

   Purpose

   expresses RSL1D1 protein in mammalian cells (puro resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   vector's LTR

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAPTrap-Clover-Muro

   Plasmid

   #82334

   Purpose

   Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   Clover-T2AMuro
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAPTrap-eGFP-IRESMeo

   Plasmid

   #82502

   Purpose

   Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   eGFP-IRESMeo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV40-EF1a-hBCL10(1-114)-SO

   Plasmid

   #75257

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Mutation

   AS 1-116 (CARD-domain)

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RKS-Fzd7/8 subtype NGS Wnt

   Plasmid

   #159628

   Purpose

   Express Fzd7/8 subtype NGS Wnt in mammalian cells

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt
   

   Use

   Lentiviral

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGBacmam-NGS Wnt-Fc

    Plasmid

    #159629

    Purpose

    Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7/8 subtype NGS Wnt Fc
    

    Use

    Tags

    Fc tag and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. RKS-Fzd7 subtype NGS Wnt

    Plasmid

    #159626

    Purpose

    Express Fzd7 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd7 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-Fzd4 subtype NGS Wnt

    Plasmid

    #159625

    Purpose

    Express Fzd4 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd4 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. RKS-Fzd8 subtype NGS Wnt

    Plasmid

    #159627

    Purpose

    Express Fzd8 subtype NGS Wnt in mammalian cells

    Depositor

    Chris Garcia

    Article

    Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

    Insert

    Fzd8 subtype NGS Wnt
    

    Use

    Lentiviral

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmiRA1-MYC

    Plasmid

    #141121

    Purpose

    Lentiviral overexpression of MYC in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB-C5

    Plasmid

    #28213

    Purpose

    5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac LIC cloning vector (4A)

    Plasmid

    #30111

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJBL7058

    Plasmid

    #167227

    Purpose

    overexpression of orthogonal T7 RNAP

    Depositor

    Julius Lucks

    Insert

    J23119-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJBL7069

    Plasmid

    #167238

    Purpose

    malachite green aptamer tagged sfGFP

    Depositor

    Julius Lucks

    Insert

    J23119-sfGFP-MGA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJBL7070

    Plasmid

    #167239

    Purpose

    malachite green aptamer tagged sfGFP

    Depositor

    Julius Lucks

    Insert

    pT7-sfGFP-MGA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL7055

    Plasmid

    #167224

    Purpose

    orthogonal T7 RNAP sfGFP reporter

    Depositor

    Julius Lucks

    Insert

    pRV-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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