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pUT1856-HQ858795

Plasmid

#27877

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

Trihelix transcription factor from DT943786

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1860-HQ858796

Plasmid

#27878

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE017439

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1862-HQ858797

Plasmid

#27879

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DR955211

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1863-HQ858798

Plasmid

#27880

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

OFP transcription factor from DV526477

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1886-HQ858799

Plasmid

#27881

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

MYB transcription factor from DR793321

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1887-HQ858800

Plasmid

#27882

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ORPHAN transcription factor from DR828861

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1888-HQ858801

Plasmid

#27883

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

ORPHAN transcription factor from EB707293

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1103-HQ858667

Plasmid

#27713

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

Trihelix transcription factor from DR821424

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-GW1D1aMut(W>A)

Plasmid

#21522

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503, mutate every tryptophan (total…

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSL1425 (pSPIN, pCDF backbone)

Plasmid

#160735

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1142 (pSPIN, pBBR1 backbone)

Plasmid

#160730

Purpose

Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pME-3x-FLAG-Ubiquitin (JDW 1098)

Plasmid

#224513

Purpose

A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3x-FLAG-Ubiquitin

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCardinal (JDW 1418)

Plasmid

#242553

Purpose

Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCardinal

Use

Gateway subcloning

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5

Plasmid

#81062

Purpose

Gateway entry vector encoding full length UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)

Use

Cloning

Mutation

K503R

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A10

Plasmid

#132275

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A10 (SLC39A10 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Mutation

See Depositor Comments Below

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176134

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Expression

Bacterial

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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