We narrowed to 14,384 results for: NTS;

1. pLVX-Puro-TDP-43-WT

   Plasmid

   #133753

   Purpose

   Wild type human TDP-43 in pLVX-Puro lentiviral vector

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a-mMLH1dn

   Plasmid

   #174825

   Purpose

   Mammalian expression of mouse MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   mouse MLH1dn (codon optimized)
   

   Expression

   Mammalian

   Mutation

   deletion of residues 758-760

   Promoter

   EF1a

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Tags

   mStayGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

   Plasmid

   #198337

   Purpose

   PB plasmid expressing Halo-tagged PP7 coat protein

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   PP7 coat protein 3x Halo-GB1
   

   Use

   Piggybac

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ173

   Plasmid

   #99907

   Purpose

   Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Insert

   pco-dCas9-VP64-T2A-MS2-VP64
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-B2 (GB3374)

   Plasmid

   #160636

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-B3omega1 (GB1694)

   Plasmid

   #160643

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

   Depositor

   Juan Antonio García, Fabio Pasin

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Drp1K38A

    Plasmid

    #45161

    Depositor

    Alexander van der Bliek, Richard Youle

    Article

    Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    HA (AYPYDVPDY)

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

    Plasmid

    #71237

    Purpose

    Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A GFP
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-hACE2-LgBiT

    Plasmid

    #215837

    Purpose

    Expresses hACE2 receptor with LgBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    hACE2-LgBiT (ACE2 )
    

    Expression

    Mammalian

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4_CRE-CMVmin-luc2

    Plasmid

    #194384

    Purpose

    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    6xCRE-CMVmin-luc2
    

    Expression

    Mammalian

    Promoter

    CMV minimal

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. QQR1 variant

    Plasmid

    #138564

    Purpose

    Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    QQR1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-7xHis-TEV-hexa

    Plasmid

    #222868

    Purpose

    Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

    Depositor

    Nikolaus Loening

    Article

    Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

    Insert

    TEV protease
    

    Tags

    7xHis and MBP

    Expression

    Bacterial

    Mutation

    C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5_EGFP-BRD4-NUT

    Plasmid

    #238254

    Purpose

    For overexpression of EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-BRD4-NUT
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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