We narrowed to 28,450 results for: tat
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Plasmid#172699PurposeUbiquitous expression of 60meric GEM monomerDepositorInsertmonomer of 60mer GEM
UseUnspecifiedTagsGFPExpressionPlantPromoterUBC1Available SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Nectin-2
Plasmid#170295PurposeA knock-out vector for the mouse Nectin-2DepositorInsertA gRNA targeting the mouse Nectin-2 gene.
UseCRISPR and LentiviralAvailable SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2bleo-Necl-5
Plasmid#170294PurposeA knock-out vector for the mouse Necl-5DepositorInsertA gRNA targeting the mouse Necl-5 gene.
UseCRISPR and LentiviralAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alanineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB-RM
Plasmid#168476PurposeN-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertN-fragment of DiB-RM
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dcp2C Δ5H-H1
Plasmid#163584Purposeexpress Dcp2 FL-H1 at endogenous level in S.cerevisiaeDepositorInsertDcp2 (DCP2 Budding Yeast)
TagsGFPExpressionYeastMutationTruncation of aa1-326; the first HLM (EDQLKSYAEEQ…PromoterDCP2 promoterAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
TagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNTI763 p(Z)-mCherry + UBC9 sgRNA
Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRExpressionYeastPromoterP(Z), synthetic promoterAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPIC_pet28a
Plasmid#131654PurposeBacterial expression of ETS gene SPICDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N0
Plasmid#160983PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1-2A
Plasmid#160986PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2-2A
Plasmid#160987PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2
Plasmid#160985PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1
Plasmid#160984PurposeExpress a fragment of mouse Pola1 proteinDepositorAvailable SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAmreB
Plasmid#149567PurposemreB gRNA expression in B. burgdorferiDepositorInsertsgRNAmreB
UseCRISPRExpressionBacterialPromoterPsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
Tags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2
Plasmid#91802PurposeExpresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
ExpressionMammalianPromoterCMVAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only