We narrowed to 17,786 results for: Met;

1. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_ST-SBP-tagBFP

   Plasmid

   #65266

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. peCALWY-6

   Plasmid

   #22238

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY6 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C416S mutation Identical to peCALWY-4, but linke…

   Promoter

   Available since

   Jan. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ii-Str_ST-SBP-mCherry

   Plasmid

   #65269

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbR-HIS-Xyl-DocI

   Plasmid

   #58711

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Xyl-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Threonin 120 to Cysteine in Xylanase Doma…

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_TNF-SBP-mCherry

   Plasmid

   #65283

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Lentiviral

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN-X-hPGK-PuroR-Z

   Plasmid

   #218904

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   hPGK-PuroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   246T>A and 398T>C

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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