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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 77,456 results for: Mycs;

Showing: 6941 - 6960 of 77456 results

  1. pYPQAT28

    Plasmid
    #223400
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223400-plasmid-map-sequence-id-452334

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  2. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  3. pEW107

    Plasmid
    #232796
    Purpose
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    ySet1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232796-plasmid-map-sequence-id-465733

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  4. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  5. pKSEe401R

    Plasmid
    #218532
    Purpose
    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.
    Depositor
    Kirill Demchenko
    Article
    Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    218532-plasmid-map-sequence-id-458122

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  7. pWR76

    Plasmid
    #202776
    Purpose
    episomal fluorescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202776-plasmid-map-sequence-id-417311

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  8. pWR23

    Plasmid
    #202770
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202770-plasmid-map-sequence-id-417278

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  9. pWR9

    Plasmid
    #202645
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202645-plasmid-map-sequence-id-409735

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  16. Ypq2-2GFP-URA

    Plasmid
    #207010
    Purpose
    Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    YPQ2
    Use
    Tags
    2xGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207010-plasmid-map-sequence-id-422256

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  17. Vph1-2GFP-URA

    Plasmid
    #207009
    Purpose
    Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Zhiping Xie
    Article
    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.
    Insert
    VPH1
    Use
    Tags
    2xGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207009-plasmid-map-sequence-id-422255

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  20. pBCC101

    Plasmid
    #202227
    Purpose
    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker
    Depositor
    Paul Schulze-Lefert
    Article
    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.
    Insert
    pBCC25, pBCC32, pBCC59, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    202227-plasmid-map-sequence-id-406226

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Showing: 6941 - 6960 of 77456 results