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pND004

Plasmid

#200950

Purpose

MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pNM58

Plasmid

#128409

Purpose

System with AIP and xylose inducible agrCA sensor

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Inserts

argCA
gfpmut2

Expression

Bacterial

Promoter

P3 and PxylR

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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EasyFusion eGFP

Plasmid

#112848

Purpose

General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

EGFP

Use

Unspecified; General donor

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-Neo

Plasmid

#48993

Purpose

Gateway adapted transfer of Neomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

aminoglycoside 3' phosphotransferase

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMyBADC

Plasmid

#84688

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Bacterial expression vector

Tags

His6

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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iRFP-N1

Plasmid

#54787

Purpose

Localization: N1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Tags

iRFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Integrin-Beta3-N-18

Plasmid

#57391

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta3

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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GG-dest

Plasmid

#69538

Purpose

Destination plasmid for gRNA concatenation Golden Gate cloning

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Type

Empty backbone

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptet1

Plasmid

#218360

Purpose

Empty backbone with tetR-PtetA expression system with single tetR operator site

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-GFP

Plasmid

#164799

Purpose

Expression of GFP in oomycetes using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

GFP

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSCR-YFP-GW

Plasmid

#209366

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWOL-YFP-GW

Plasmid

#209365

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET11b-HA2

Plasmid

#165992

Purpose

Influenza HA ministem with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

HA2

Tags

Foldon and HisTag

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL815

Plasmid

#52322

Purpose

A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.

Depositor

Naweed Naqvi

Article

Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10

Type

Empty backbone

Use

Agrobacterium

Expression

Bacterial

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-COF1

Plasmid

#31844

Depositor

David Drubin

Article

Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

Insert

Cofilin (COF1 Budding Yeast)

Tags

GFP (S65T)

Expression

Yeast

Promoter

T7

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
p5055

Plasmid

#168178

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PhtrA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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NK803

Plasmid

#166057

Purpose

Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Inserts

Pac puromycin resistance gene
LifeAct-mCherry

Use

S. rosetta expression

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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