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Showing: 3481 - 3490 of 170787 results

  1. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

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  8. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  10. pAAV-synmiR-L-mRuby-Pro-EGFR-mEGFP-4×18

    Plasmid
    #218266
    Purpose
    Expresses EGFR-mEGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFR-mEGFP-MREL_4×18
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218266-plasmid-map-sequence-id-494337

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Showing: 3481 - 3490 of 170787 results