161,873 results

1. CMV-OMM-jRCaMP1b

   Plasmid

   #127871

   Purpose

   Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHSE401

   Plasmid

   #62201

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-iGABASnFR2n-WPRE

   Plasmid

   #218875

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   Synapsin

   Available since

   May 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-p25

   Plasmid

   #24896

   Depositor

   Harish Pant

   Article

   Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

   Insert

   p25 (CDK5R1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal truncated version of p35, contains aa …

   Promoter

   Available since

   May 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. TC374

   Plasmid

   #164886

   Purpose

   Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   rpl28-rtTA(Q)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRVL-1

   Plasmid

   #104579

   Purpose

   Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse CL kappa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-EGFP

   Plasmid

   #27082

   Purpose

   Integration-free (episomal) expression of EGFP

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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