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pBHM2329

Plasmid

#171686

Purpose

pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

Depositor

Hiten Madhani

Article

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

C. neoformans U6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM9037

Plasmid

#153225

Purpose

Template for PCR amplification of the constant part of guide RNAs

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

guide RNA constant part, carotenoid marker

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO MYC3

Plasmid

#177848

Purpose

Bacterial Expression of MYC3

Depositor

Karsten Melcher

Article

Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

Insert

MYC3 (MYC3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCaRNA1&2

Plasmid

#86087

Purpose

Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

Depositor

Aiming Wang

Article

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

RNA1, RNA2

Expression

Plant

Promoter

35S

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCaRNA3

Plasmid

#86088

Purpose

Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

Depositor

Aiming Wang

Article

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

RNA3

Expression

Plant

Promoter

35S

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRSetB-KanR-LiLac

Plasmid

#184569

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

Depositor

Gary Yellen

Article

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Insert

LiLac fluorescent sensor of lactate

Tags

His7

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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miRFP670-TRF1

Plasmid

#227388

Purpose

Label telomere, no adhesion

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric miRFP (TERF1 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Cytosolic Syk Biosensor

Plasmid

#125729

Purpose

FRET biosensor for studying SYK activities in cells

Depositor

Yingxiao Wang

Article

Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

Insert

SYK biosensor (ZAP70 Synthetic)

Tags

6xHistine Tag at the N-terminal.

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pNCS mStrawberry

Plasmid

#91768

Purpose

Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Strawberry

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pVAX1/mTyr-Cre

Plasmid

#99249

Purpose

Expresses Cre recombinase under tyrosinase promoter

Depositor

Christian Blank

Article

Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

Inserts

Cre recombinase
mTyr enh/prom

Tags

Myc

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-TCAB1

Plasmid

#51630

Purpose

Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

TCAB1 (WRAP53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) HIPK3 Sense

Plasmid

#60634

Purpose

Expresses the human HIPK3 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Homeodomain-interacting protein kinase 3 (HIPK3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP8H

Plasmid

#188062

Purpose

PROSS-designed versatile peroxidase 8H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcHisB-TRF2

Plasmid

#50488

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-500

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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