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LeGO-Y

Plasmid

#27357

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, eYFP

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

Plasmid

#197066

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

Depositor

Julia Mahamid

Publication

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Use

CRISPR

Tags

FKBP and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE)

Plasmid

#140226

Purpose

Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance

Depositor

Stanley Qi

Publication

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

Luciferase + crIFNg

Expression

Mammalian

Promoter

TRE3G

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Expression

Mammalian

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a-EGFP

Plasmid

#205307

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

Depositor

Eric Schreiter

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hSyn1_AchLightG

Plasmid

#187464

Purpose

Expresses green acetylcholine indicator AchLightG in neuronal cells

Depositor

Tommaso Patriarchi

Publication

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AchLightG

Use

AAV

Tags

Igκ leader

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII-tdTomato

Plasmid

#162752

Purpose

Retroviral (MSCV) expression of td Tomato fluorescent protein

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

tdTomato

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1433

Plasmid

#129523

Purpose

pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Publication

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Publication

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Expression

Worm

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNXRVa-HACre

Plasmid

#78398

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-GFP no-pA

Plasmid

#80813

Purpose

GFP without pA

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP no pA

Expression

Mammalian

Promoter

GFP

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pIM690

Plasmid

#74638

Purpose

alias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection marker

Depositor

Jiří Hašek

Publication

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHyVec12

Plasmid

#51851

Purpose

Expression in Hydra for RNAi

Depositor

Haifan Lin

Publication

Juliano et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

Inserts

GFP hairpin
DsRed2

Use

RNAi; Hydra expression

Promoter

Actin

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAM1706

Plasmid

#40242

Depositor

Susan Golden

Publication

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

PpsbAI

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCitrine

Plasmid

#34951

Depositor

Amro Hamdoun

Publication

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pXN-FBLWLF

Plasmid

#26262

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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TS245_pNKI-2-13_EGFP-DPN7

Plasmid

#200719

Purpose

producing Methyl Tracer EGFP protein in bacteria

Depositor

Bas van Steensel

Publication

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

eGFP-DPN7

Tags

6xHis

Expression

Bacterial

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HB

Plasmid

#184868

Purpose

pSwap plasmid part with HB homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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