Skip to main content
Addgene
Showing: 1361 - 1400 of 5000 results

  1. pLV-NT-3-mCherry

    Plasmid
    #190201
    Purpose
    To express the mouse NT-3 tagged with the FP mCherry
    Depositor
    Pantelis Tsoulfas
    Insert
    NT-3
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190201-plasmid-map-sequence-id-374466

    Enlarge

  2. SRPK2

    Plasmid
    #39047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK2 (SRPK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Internal segment 268-518 deleted; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pHH0103 NEDD4L WW domain #2

    Plasmid
    #104205
    Purpose
    Bacterial expression of WW domain #2 from NEDD4L
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4L WW domain #2 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104205-plasmid-map-sequence-id-203673

    Enlarge

  4. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pHH0103 NEDD4L WW domain #3

    Plasmid
    #104206
    Purpose
    Bacterial expression of WW domain #3 from NEDD4L
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4L WW domain #3 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104206-plasmid-map-sequence-id-202575

    Enlarge

  6. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

    Enlarge

  7. UXS1

    Plasmid
    #39162
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UXS1 (UXS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pHH0103 NEDD4L WW domain #1

    Plasmid
    #104204
    Purpose
    Bacterial expression of WW domain #1 from NEDD4L
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4L WW domain #1 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104204-plasmid-map-sequence-id-203479

    Enlarge

  9. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

    Plasmid
    #160618
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160618-plasmid-map-sequence-id-322016

    Enlarge

  10. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

    Enlarge

  11. pHH0103 NEDD4L WW domain #4

    Plasmid
    #104207
    Purpose
    Bacterial expression of WW domain #4 from NEDD4L
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    NEDD4L WW domain #4 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104207-plasmid-map-sequence-id-202579

    Enlarge

  12. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211337
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211337-plasmid-map-sequence-id-415646

    Enlarge

  14. pET3-ARL9(CO)

    Plasmid
    #201873
    Purpose
    Used for bacterial expression of human protein ARL9
    Depositor
    Richard Kahn
    Insert
    ARL9 (ARL9 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    201873-plasmid-map-sequence-id-403806

    Enlarge

  15. pET3-ARF4-His6(CO)

    Plasmid
    #201885
    Purpose
    Used for bacterial expression of human protein ARF4
    Depositor
    Richard Kahn
    Insert
    ARF4 (ARF4 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201885-plasmid-map-sequence-id-403153

    Enlarge

  16. pET3-ARF5-His6(CO)

    Plasmid
    #201886
    Purpose
    Used for bacterial expression of human protein ARF5
    Depositor
    Richard Kahn
    Insert
    ARF5 (ARF5 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201886-plasmid-map-sequence-id-403150

    Enlarge

  17. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid
    #160640
    Purpose
    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).
    Depositor
    Diego Orzaez
    Insert
    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160640-plasmid-map-sequence-id-321670

    Enlarge

  18. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104297-plasmid-map-sequence-id-203688

    Enlarge

  30. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid
    #92102
    Purpose
    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92102-plasmid-map-sequence-id-197631

    Enlarge

  32. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

    Enlarge

Showing: 1361 - 1400 of 5000 results