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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 681 - 700 of 2967 results

  1. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc

    Plasmid
    #196237
    Purpose
    Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.
    Insert
    Af1521(K35E/Y145R) encoding a C-terminal myc tag
    Use
    Lentiviral
    Tags
    myc tag
    Expression
    Mammalian
    Mutation
    amino acid 35 lysine is replaced with glutamic ac…
    Promoter
    EF1A
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    196237-plasmid-map-sequence-id-398172

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  10. LSB-hsa-miR-543

    Plasmid
    #103623
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-543 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-543 target (MIR543 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103623-plasmid-map-sequence-id-196294

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  19. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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Showing: 681 - 700 of 2967 results