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C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdTomato-WPRE

Plasmid

#104105

Purpose

Expresses tdTomato under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTK372

Plasmid

#114687

Purpose

sgRNA targeting NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA sgRNA

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx4

Plasmid

#104472

Purpose

Integrating plasmid for overexpression of OM45-FKBPx4.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx4- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTK398

Plasmid

#114714

Purpose

mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

mAID-mClover-3FLAG

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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JB26

Plasmid

#128920

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab33BQL-BirA*-HA-MAO (RAB33B Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTK711

Plasmid

#114708

Purpose

Express NuMA(1-1985)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-1985)-RFP-Nano

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDM767

Plasmid

#207425

Purpose

Extrachromosomal expression HSPC300-GFP and PH-CRAC-mRFPmars

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
CRAC

Use

Unspecified

Tags

GFP and mRFPmars

Mutation

aa 1–126 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1424

Plasmid

#207424

Purpose

Extrachromosomal expression HSPC300-GFP and PkgE-PH-mCherry

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
PkgE

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–100 (PH domain) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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JB15

Plasmid

#128918

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab30QL-BirA*-HA-MAO (RAB30 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pX49

Plasmid

#128914

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab11BQL-BirA*-HA-MAO (RAB11B Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx2

Plasmid

#104471

Purpose

Integrating plasmid for overexpression of OM45-FKBPx2.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx2- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316 GAL cluster

Plasmid

#61925

Purpose

Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316

Depositor

Jon Houseley

Article

Cruz et al Elife. 2014 Feb 11;3:e01581. doi: 10.755

Inserts

GAL1 (GAL1 Budding Yeast)
GAL10 (GAL10 Budding Yeast)

Expression

Yeast

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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JB3

Plasmid

#128919

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab30TN-BirA*-HA-MAO (RAB30 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1a-jAspSnFR3-mRuby3

Plasmid

#203491

Purpose

jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3

Use

Gateway entry vector

Tags

His-tag and mRuby3

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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JB25

Plasmid

#128921

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab33BTN-BirA*-HA-MAO (RAB33B Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB88

Plasmid

#128933

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebSN-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB87

Plasmid

#128932

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

RhebQL-BirA*-HA-MAO (RHEB Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTK746

Plasmid

#114712

Purpose

Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(1-2115ΔNLS)5A-3-RFP-Nano

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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