We narrowed to 18,897 results for: Met;
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Plasmid#217999PurposeStart-methionineDepositorInsertStart-Met
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
289aa ELL2
Plasmid#127266PurposeFor in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.DepositorInsertNH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to Ileu. Contains 289…PromoterT7Available SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK-Ma pylRS nitroY/haloY-F5
Plasmid#212125PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS 3-NY/HaloY F5
ExpressionBacterialMutationCTG125CTT, N166A V168S, A223C, W239RPromoterGlnS (constitutive)Available SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ECFPEYFP
Plasmid#24520DepositorInsertECFP, EYFP
TagsECFP and EYFPExpressionMammalianAvailable SinceJune 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
TagsHis6 and SUMOExpressionBacterialPromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p416GPD-PYK1
Plasmid#34604DepositorInsertPYK1
ExpressionYeastPromoterGPDAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
P413TEF-PYK1
Plasmid#34605DepositorInsertPYK1
ExpressionYeastPromoterTEFAvailable SinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PYK2
Plasmid#34606DepositorAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB12720
Plasmid#212704PurposegRNA targeting to the gene 4HPPD locusDepositorInsertgRNA targeting to the gene 4HPPD locus
ExpressionYeastAvailable SinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-AP-1-mCherry
Plasmid#118095PurposeFluorescent reporter for AP-1 activationDepositorInsertmCherry
UseRetroviralPromoterminimal promoter with AP-1 responsive elementAvailable SinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NFAT-eGFP
Plasmid#118031PurposeFluorescent reporter for NFAT activationDepositorInserteGFP
UseRetroviralPromoterminimal promoter with NFAT binding siteAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA-BSD
Plasmid#175583PurposeExpresses sgRNA for CasRx in mammalian cellDepositorInsertsgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eCFP
Plasmid#118094PurposeFlourescent reporter for NF-kB activityDepositorInserteCFP
UseRetroviralPromoterminimal promoter with NF-kB responsive elementAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)- PC-MjMAT
Plasmid#179372PurposePC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.DepositorInsertPC-MjMAT
Tags6xHisExpressionBacterialMutationL147A, I351APromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-TAG35-sfGFP
Plasmid#212120PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-TAG35-sfGFP
M. alvus Pyl-tRNA
TagsHis6 and SUMO-TAG35ExpressionBacterialMutationE35TAG (within SUMO encoding sequence)PromoteraraC and lppAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK Mma PylT (CUA)
Plasmid#154782Purposeplasmid with single copy of Mma PylT (CUA anticodon)DepositorInsertMma PylT
ExpressionMammalianMutationU25CPromoterh7SKAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SBC015910
Plasmid#226287PurposeExpresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.DepositorInsertsConiferyl alcohol acyltransferase
Isoeugenol synthase 1
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only