We narrowed to 10,761 results for: T7

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pTM1_VP35_ZEBOV

Plasmid

#68121

Purpose

Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP35 ZEBOV (VP35 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTM1_VP30_ZEBOV

Plasmid

#69119

Purpose

Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP30 ZEBOV (VP30 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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p2,0_3E5E_luciferase

Plasmid

#69358

Purpose

Encodes for Ebola Minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

3E5E luciferase minigenome

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pTM1_L_ZEBOV

Plasmid

#69120

Purpose

Encodes for Ebola Virus Zaire L under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

L ZEBOV (L )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site1 (RTW443)

Plasmid

#160136

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site4 (MSP3511)

Plasmid

#160139

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJin-290

Plasmid

#125718

Purpose

ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C

Depositor

Bryan Dickinson

Article

Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

Insert

PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB

Expression

Mammalian

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pr1-T7RNAP

Plasmid

#67738

Purpose

OR2-OR1-Pr1 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

Plasmid

#228508

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I5CHO (#1)

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB578_pB1H1_T7-RNAP_LARP-I

Plasmid

#228507

Purpose

Bacterial Hybrid Driver Plasmid

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

T7 RNAP, LARP-I

Use

Synthetic Biology

Tags

Umu Degron, GTG-start codon

Mutation

R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-5'TwStrep

Plasmid

#208620

Purpose

Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

Depositor

Quanfu Ma

Type

Empty backbone

Tags

Twin Strep

Expression

Bacterial

Promoter

T7

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

Plasmid

#48307

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGBR22-pGEM-T

Plasmid

#231055

Purpose

Expresses Montipora efflorescens GFP-like chromoprotein under T7 with a 6xHIS tag

Depositor

Josh Beckham

Insert

GFP-like chromoprotein

Use

Cloning

Tags

6x HIS

Promoter

T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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