Addgene

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pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLYS5-MCU-Flag

Plasmid

#50054

Purpose

Expression of human MCU in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

MCU-Flag (MCU Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pDuex2-iBB-GECO1

Plasmid

#194216

Purpose

Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

Depositor

Robert Campbell

Article

Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

Insert

iBB-GECO1

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLYS1-EMRE/RNAi resistant

Plasmid

#50056

Purpose

Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

EMRE-RNAi resistant (SMDT1 Human)

Use

Tags

Expression

Mammalian

Mutation

RNAi resistant, silent mutant

Promoter

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-iBB-GECO1

Plasmid

#194215

Purpose

Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

Depositor

Jin Zhang

Article

Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

Insert

iBB-GECO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8f

Plasmid

#169258

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

Plasmid

#111925

Purpose

Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU

Plasmid

#31726

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19. ():.

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

*

Promoter

None

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6f

Plasmid

#112000

Purpose

For enriched expression of GCaMP6f in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV1

Insert

axon-GCaMP6f

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-4mtD3cpv

Plasmid

#181874

Purpose

Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

Insert

4mtD3cpv

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

the 4mtD3cpv CDS differs from the recorded GeneBa…

Promoter

CaMKIIa

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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MUNC18-2

Plasmid

#186687

Purpose

Expresses MUNC18-2 in insect cells

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

MUNC18-2 (Stxbp2 Rat)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-23

Plasmid

#186686

Purpose

Expresses SNAP-23 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

SNAP23

Use

Tags

GST and thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

Plasmid

#163881

Purpose

Acute Depletion of Mau2-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CAD

Plasmid

#73567

Purpose

Soluble activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

CAD (STIM1 342-448) (STIM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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px330 NIPBL gRNA

Plasmid

#165590

Purpose

Insertion of NIPBL degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

NIPBL (Nipbl Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6m

Plasmid

#111261

Purpose

For enriched expression of GCaMP6m in axons

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6m

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-hBIN1-V5

Plasmid

#27303

Depositor

Robin Shaw

Article

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Insert

bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only