159,481 results

1. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCap-101-Apr

   Plasmid

   #227507

   Purpose

   Capture plasmid with apramycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Pol-IIΔ

   Plasmid

   #35176

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the entire C-terminal domain (repeats…

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEVA451-Cpf1

   Plasmid

   #192362

   Purpose

   CRISPR-based vector for genome editing

   Depositor

   Juana María Navarro Llorens

   Article

   Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

   Insert

   Cas12a, gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-EGFPL10a

   Plasmid

   #98747

   Purpose

   Cre-dependently expresses the fusion protein EGFPL10a

   Depositor

   Nathaniel Heintz, Alexander Nectow, Eric Schmidt

   Article

   Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

   Has Service

   AAV Retrograde and AAV5

   Insert

   EGFPL10a
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xNLS-pMJ915v2

   Plasmid

   #88917

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-JMJD3

   Plasmid

   #24167

   Purpose

   Mammalian expression of human JMJD3 with HA tag

   Depositor

   Kristian Helin

   Article

   Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40

   Plasmid

   #98928

   Purpose

   Cre dependent AAV expression of Ruby2 spaghetti monster FLAG construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Has Service

   AAV1

   Insert

   Ruby2-smFP-Flag
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB2F

   Plasmid

   #195289

   Purpose

   Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFR WT

    Plasmid

    #11011

    Purpose

    Retroviral construct for expressing human EGFR in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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