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Plasmid#225193PurposeUsed for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag003
Plasmid#225194PurposeUsed for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag003
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP103: pTarget(CyCAST)
Plasmid#200849PurposeTarget plasmid containing CyPSP1 and attachement site for CyCAST.DepositorInsertsPAM for CyCAST + CyPSP1
tRNA-Leu
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-WPRE-bGH-DEST (JDW 1309)
Plasmid#229815PurposeA CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcriptsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBeta-MHC-promoter DV (JDW 26)
Plasmid#229835PurposeA gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-DV-IRES-myr-BFP (JDW 491)
Plasmid#229834PurposeA CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membraneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
SBC015869
Plasmid#226281PurposeExpresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.DepositorInserts4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGK Promoter (MTK 2) (pAN2822)
Plasmid#194224PurposeMammalian Toolkit part 2 encoding the PGK promoterDepositorInsertPGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterPGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a Promoter (MTK 2) (pAN2824)
Plasmid#194225PurposeMammalian Toolkit part 2 containing the EF1a promoterDepositorInsertEF1a Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-mCherry-WzzE
Plasmid#196663PurposeExpression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
UseTagsHis and mCherryExpressionBacterialMutationValine insertion at position 2 of WzzE.PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPglL-strep-mCherry
Plasmid#196664PurposeExpression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coliDepositorInsertPglL
UseTagsTEV-cleavable distrep tag II and mCherryExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR064
Plasmid#185031Purpose400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR074-control-sfGFP
Plasmid#185032PurposeJ23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR102
Plasmid#185033Purpose400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationU54C mutation of rpmE 5'UTR, Only first 90 n…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR106
Plasmid#185036Purpose400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG79U mutation of rpmE 5'UTR, Only first 90 n…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR116
Plasmid#185040Purpose400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationU58C and U70C of rpmE of 5'UTR, Only first 9…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR017-L31-sfGFP
Plasmid#185024Purpose400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationOnly first 90 nt of rpmE coding presentPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-mRuby3
Plasmid#166142PurposeGateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.DepositorArticleTypeEmpty backboneUseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only