We narrowed to 7,762 results for: loxP
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Plasmid#211055PurposeMammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.DepositorInsertCOPB2:M1-A597 (COPB2 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB2-hPER1-Halo-CD4-bla
Plasmid#189982PurposeDonor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion proteinDepositorInsertHaloTag
UseCRISPRTags3xFlagAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-NPHP1
Plasmid#209769PurposeHuman NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertNPHP1 (NPHP1 Human, Synthetic)
UsePiggybac transposonTagsHA tagExpressionMammalianPromoterCAGAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcb306-GFP-Rab12-mito
Plasmid#207349PurposeMammalian expression of Rab12-mitoDepositorInsertRab12-mito (RAB12 Human)
ExpressionMammalianAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_g5-HT2h
Plasmid#208715PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinaseDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
UseAAVPromoterhSynAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_CRF0.5
Plasmid#208660PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-mSox17FNV-3xflag
Plasmid#206375PurposeExpresses 3xflag fused mouse SOX17FNV in mammalian cells, for lentivirus generation.DepositorPublicationAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-mSox2C17
Plasmid#206369PurposeExpresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.DepositorPublicationAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMDS 2 colors
Plasmid#206265PurposeExpression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD
Plasmid#196878PurposeNeuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.DepositorInsertmOrange2-N-gTBD
UseCre/LoxExpressionMammalianPromoterQuimeric CAGAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro
Plasmid#201677PurposeEBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassetteDepositorInsertMIR302-3g-EEA-2g-PGK-Puro
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1
Plasmid#196891PurposeNeuron-specific expression of human (h)Centrin fused to 2xHA-tagsDepositorAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]
Plasmid#196884PurposeDual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-Lyn-GFP-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196886PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…ExpressionInsectPromoterU6-3Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-intron-DIO-hfCas13d-pA
Plasmid#195866PurposeTo express hfCas13dDepositorInserthigh fidelity (hf) version of CasRx (hfCas13d)
UseAAVMutationYes: GTGGAATACATTACCAACGTGGTGTACGTGPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only