We narrowed to 7,762 results for: loxP

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double_sgRNA targeting e1 and e4

Plasmid

#190688

Purpose

sgRNAs targeting enhancer 1 and 4 of MYC separately

Depositor

Stanley Qi

Publication

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 1 and 4 of MYC separately (MYC Human)

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5004_hU6_sgRNA targeting e7

Plasmid

#190687

Purpose

sgRNA targeting enhancer 7 of MYC

Depositor

Stanley Qi

Publication

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 7 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Promoter

Human U6 promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2196

Plasmid

#170400

Purpose

For expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-717, replaced with 114-130)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2197

Plasmid

#170401

Purpose

For expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 347-360)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2198

Plasmid

#170402

Purpose

For expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ702-716, replace with 580-600)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2251

Plasmid

#170403

Purpose

For expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 225-249)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2252

Plasmid

#170404

Purpose

For expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-716, replace with 455-479)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2253

Plasmid

#170405

Purpose

For expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Insert

LetAB (letB, letA E. coli)

Tags

8xHis2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

LetA-LetB(Δ700-717, replace with 812-836)

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0-EGFP-HRasC20

Plasmid

#187252

Purpose

Lenticiral expression of EGFP-HRasC20

Depositor

Alpha Yap

Publication

Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi:

Insert

HRasC20 (HRAS Human)

Use

Lentiviral

Tags

EGFP

Promoter

5' LTR

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM262 - pDESTR4-R3 mosTI hygroR target

Plasmid

#159840

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI hygroR target

Expression

Worm

Mutation

Not applicable

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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AiP1069-Ai210 (TITL-GC7f-ICF-IRES-tTA2)

Plasmid

#181763

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

GCaMP7f and tTA2

Expression

Mammalian

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)

Plasmid

#170488

Purpose

SPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1

Depositor

Klaus Hahn

Publication

Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

Insert

FRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry

Use

Retroviral

Expression

Mammalian

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin(N229A)-TEV-His6

Plasmid

#178077

Purpose

Insect cell expression of gamma-tubulin(N229A)-TEV-His6

Depositor

Tarun Kapoor

Publication

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin with an N229A point mutation (TUBG1 Human)

Tags

TEV-His6

Expression

Insect

Mutation

Changed Asparagine 229 to Alanine

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_MAP7_N

Plasmid

#159728

Purpose

Expresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Publication

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

MAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)

Tags

ZZ affinity tag and ybbR tag

Expression

Insect

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2E3E4 PUb-DsRed

Plasmid

#173665

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Publication

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
DsRed

Expression

Insect

Mutation

Nix isoforms with 4 exons

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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RAP2A-p2A-E2Crimson

Plasmid

#83940

Purpose

Lentiviral vector expressing RAP2A-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SLC35B2-p2A-E2Crimson

Plasmid

#83941

Purpose

Lentiviral vector expressing SLC35B2-p2A-E2Crimson

Depositor

David Sabatini

Publication

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

SLC35B2 (SLC35B2 Human)

Use

Lentiviral

Tags

p2A-E2Crimson

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

Plasmid

#159907

Purpose

Mutagenesis of Netrin1

Depositor

Larry Zweifel

Insert

Netrin1 (Ntn1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045E

Plasmid

#125007

Purpose

Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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