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pYPQ132-STU-Lb

Plasmid

#138099

Purpose

Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-pT

Plasmid

#138108

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221 UBR5

Plasmid

#81062

Purpose

Gateway entry vector encoding full length UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)

Use

Cloning

Mutation

K503R

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ142-ZmUbi

Plasmid

#138106

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A10

Plasmid

#132275

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A10 (SLC39A10 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLB038 - PHS1∆P

Plasmid

#136285

Purpose

entry vector for GreenGate cloning method, C module, open reading frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) (PHS1DP)

Depositor

Alexis Maizel

Article

Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

Insert

PHS1∆P

Expression

Bacterial

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEntr1a-mcherry-FKBP-TIAM1(DHPH)

Plasmid

#176135

Purpose

Heterodimerization with FRB (rapamycin), Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Mutation

See Depositor Comments Below

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176134

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBSII-PGK-FLPo-IRES-Puro

Plasmid

#205989

Purpose

Expresses Flp recombinase and puromycin resistance

Depositor

Sharon Torigoe

Article

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Inserts

PGK promoter
FLPo (flp )
IRES-PuroR cassette

Expression

Mammalian

Promoter

PGK promoter

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

Plasmid

#109048

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

eGtACR1-ST

Use

AAV

Promoter

CAG

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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p2R3a-TagRFP-OcsT

Plasmid

#71270

Purpose

Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

4xGly linker and octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+-HypocratesCS

Plasmid

#161960

Purpose

Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

Depositor

Dmitry Bilan

Article

Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

Insert

Hypocrates

Expression

Mammalian

Mutation

changed Cysteine 355 to Serine

Promoter

CMV, SP6

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

min-ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-MECP2

Plasmid

#47806

Purpose

Expresses methyl-CpG binding protein 2 (Rett Syndrome)

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)

Use

Gateway entry vector

Mutation

wild type

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pME map2k2a_s219D

Plasmid

#201058

Purpose

Gateway middle entry vector expressing constitutively active zebrafish map2k2a

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

MAP2k2a (map2k2a Zebrafish)

Use

Zebrafish expression

Mutation

Mutation S219D, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR4 CDS A. thaliana PHOT1

Plasmid

#209187

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT3G45780.1 (PHOT1 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-As

Plasmid

#138103

Purpose

Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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