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pEMS1177

Plasmid

#29091

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple81 (GAL Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP

Plasmid

#192287

Purpose

Expresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

Cas9-T2A-GFP and four sgRNA: GBX2 + CDX1 + CDX2 + CDX4 (GBX2, CDX2, CDX1, CDX4 Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1590

Plasmid

#29269

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple119 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pBIG1a-PAF1C-CDC73deltaKIM

Plasmid

#231748

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

CDC73(delta290-324)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a-PAF1C-CDC73-YY/AA

Plasmid

#231749

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CTR9 (CTR9 Human)
CDC73 (CDC73 Human)
PAF1 (PAF1 Human)
LEO1 (LEO1 Human)
WDR61 (SKIC8 Human)

Tags

3C-Strep(II) and Flag-3C

Expression

Insect

Mutation

CDC73(Y290A, Y293A)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBS-GBX2-CDX124-Ef1a-mCherry-Neo

Plasmid

#192823

Purpose

Expresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

mCherry and sgRNA CDX1 CDX2 CDX4 and GBX2 (CDX2, CDX1, CDX4, GBX2 Synthetic)

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1054

Plasmid

#29006

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple118 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre-EGFP-NLS

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1056

Plasmid

#29007

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple120 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre-EGFP-NLS

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1253

Plasmid

#29129

Purpose

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple108 (HBEGF Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1580

Plasmid

#29266

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple109 (HBEGF Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 7, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1255

Plasmid

#29131

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple110 (HBEGF Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1053

Plasmid

#29005

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple117 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre-EGFP-NLS

Available Since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only

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FGFR2 3xFlag

Plasmid

#110063

Purpose

FGFR2 expression in mammalian cells (JCOPCO paper)

Depositor

Martin Fernandez-Zapico

Article

Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

Insert

fibroblast growth factor receptor 2 (FGFR2 Human)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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MIP 247 CoMiP 4in1 with shRNA p53

Plasmid

#63726

Purpose

To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Insert

Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)

Tags

IRES-dtomato

Expression

Mammalian

Mutation

codon-optimised

Promoter

SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_RAB7A:1-207

Plasmid

#211098

Purpose

Mammalian expression of full-length human RAB7A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHTN-HaloTag_RAB7A:1-207

Plasmid

#211095

Purpose

Mammalian expression of full-length human RAB7A. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-N_COP1:1-731

Plasmid

#211039

Purpose

Mammalian expression of full-length human COP1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COP1:M1-V713 (COP1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1B:1-489

Plasmid

#211196

Purpose

Mammalian expression of full-length human CORO1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1B:M1-A489 (CORO1B Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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