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pCI Flag Smad3 NL AAGY

Plasmid

#27041

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; P181A P182A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 EPSM

Plasmid

#27222

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

T179V S204A S208A S213A

Available Since

Dec. 21, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 AAGY

Plasmid

#27013

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

P181A P182A

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
HsCLYBL(aa23-340)-His

Plasmid

#111288

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) codon optimized for bacterial expression (CLYBL Human)

Tags

His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDNA5-FRT/TO-FLAG-TONSL

Plasmid

#221011

Purpose

mammalian expression vector of Flag tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

Flag

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPR v2-sgCrls1-1#

Plasmid

#234765

Purpose

knockout Crls1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCrls1-2#

Plasmid

#234766

Purpose

knockout Crls

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA G23D

Plasmid

#122926

Purpose

Bacterial expression of RALA G23D

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

G23D

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Sec61β

Plasmid

#246874

Purpose

Expression of mScarelt3-S2 fused with Sec61β. This construct is used for imaging the ER.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

Sec61β

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Mouse, Aequorea victoria)

Tags

HA-Tag and pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCE-hOCT3/4

Plasmid

#41813

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNT

Plasmid

#138681

Purpose

Expresses a non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A-Rigor

Plasmid

#172207

Purpose

Expresses KIF1A G251A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 251 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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