We narrowed to 8,596 results for: Set

1. pMC_mNG2(11)_BSDminus_F0

   Plasmid

   #184162

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC167

   Plasmid

   #118909

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-715MRPprom-MRPcucuc

   Plasmid

   #87199

   Purpose

   Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   RMRP (RMRP Human)
   

   Expression

   Mammalian

   Mutation

   GAGAG91-95 to CUCUC

   Promoter

   human RMRP

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-LivePAR(R163A)-Hygro

   Plasmid

   #187609

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Insert

   LivePAR(R163A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R163A mutation in the WWE Domain

   Promoter

   EF1A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMGS59(AID-ARF-P2A-Hygromycin)

   Plasmid

   #138174

   Purpose

   An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

   Insert

   AID-ARF-P2A-Hygromycin
   

   Tags

   AID-ARF clamp degron tag

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC031 - RcrCas13a from Rhodobacter capsulatus R121

   Plasmid

   #91921

   Purpose

   Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcrCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLib6.4-Cquin_596

   Plasmid

   #176655

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEG R1-ChlorR-R4 #BGV049

   Plasmid

   #48943

   Purpose

   Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

   Plasmid

   #91917

   Purpose

   Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LweCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRES.Puro.mBeta

    Plasmid

    #24175

    Depositor

    Robert Sobol

    Article

    Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

    Insert

    pIRES.Puro.mBeta (Polb Mouse)
    

    Expression

    Mammalian

    Mutation

    x

    Available Since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDGE671

    Plasmid

    #153237

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    GFP

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQnl_tet

    Plasmid

    #191355

    Purpose

    Clostridium expression vector with TetR-repressed NanoLuc expression

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette
    

    Expression

    Bacterial

    Promoter

    PGus2A-TetO2/1

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNAS1b split mCherry 14-3-3β

    Plasmid

    #111880

    Purpose

    Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Tags

    Split mCherry system: 14-3-3β fused to C-terminal…

    Expression

    Bacterial

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM65893

    Plasmid

    #153216

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-2Aneo

    Plasmid

    #80032

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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