We narrowed to 18,937 results for: Tec

1. pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

   Plasmid

   #199328

   Purpose

   mutated RHIM motive (interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed V458, Q459,V460 and G461 to alanine

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rescue Septin 11 mRNA

   Plasmid

   #122022

   Purpose

   Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Expression

   Mammalian

   Mutation

   Silent mutations are in 5 consecutive codons (AUU…

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. [pSK468] pLJC5 Flag-Depdc5

   Plasmid

   #109337

   Purpose

   Lenti-viral expression of Flag-tagged wildtype Depdc5

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TS MS-Sensor

   Plasmid

   #199788

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   TS MS-Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMW96

   Plasmid

   #178066

   Purpose

   Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

   Insert

   CDK5RAP2 (CDK5RAP2 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

   Plasmid

   #193469

   Purpose

   Cp36 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NG-CGBE1

   Plasmid

   #198537

   Purpose

   Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTrc99S-ssDsbA-MBP-4xDQNAT

   Plasmid

   #128398

   Purpose

   Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Maltose binding protein
   

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Y342H- please see depositor comments

   Promoter

   trc

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EFS-fDIO-GRAB_eCB2.0

    Plasmid

    #164610

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    EFS

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA179

    Plasmid

    #215939

    Purpose

    Fragmid fragment: (guide cassette) short sequence with no known function

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Filler
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA331

    Plasmid

    #215954

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-DIO-GRAB_eCB2.0mut

    Plasmid

    #164607

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. RS35075_pMQ30_KO_Kan

    Plasmid

    #185390

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    

    Expression

    Bacterial

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIMVAu1

    Plasmid

    #98301

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    

    Expression

    Yeast

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA

    Plasmid

    #205320

    Purpose

    All-in-one AAV vector expressing ISAba30 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISAba30 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid

    #169548

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKate2-P2A-APEX2-ML1N*2

    Plasmid

    #67665

    Purpose

    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    mKate2-P2A-APEX2-ML1N*2
    

    Expression

    Mammalian

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 100,50 (RAB7A)

    Plasmid

    #170117

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 100,50 guide RNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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