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pFA6a-link-yomTagBFP-SpHis5

Plasmid

#44838

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJFRC159-1XUAS-DSCP-B3::PEST

Plasmid

#32137

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Expression

Insect

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pmTUBB3

Plasmid

#31236

Depositor

Cliff Ragsdale

Publication

Grove et al Development. 1998 Jun . 125(12):2315-25.

Insert

TUBB3 (Tubb3 Mouse)

Use

Cdna for in situ hybridization

Mutation

3'UTR sequence

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-AGK

Plasmid

#23578

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AGK (AGK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Publication

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Mutation

n/a

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a-EGFP

Plasmid

#205307

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Publication

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAG460-FRB-greenNFAST

Plasmid

#160361

Purpose

Expresses FRB-greenNFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

FRB-greenNFAST

Expression

Mammalian

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSL1433

Plasmid

#129523

Purpose

pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Publication

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Publication

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Expression

Worm

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGrDL_SP

Plasmid

#83204

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Publication

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

SalI/PstI cloning sites

Expression

Plant

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTSTEP

Plasmid

#72334

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Publication

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-EGFP

Use

CRISPR

Expression

Insect

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pSUP202pol4

Plasmid

#67184

Purpose

Conjugative cloning vector

Depositor

Hauke Hennecke, Julia Vorholt

Publication

Fischer et al EMBO J. 1993 Jul;12(7):2901-12.

Type

Empty backbone

Use

Conjugative bacterial cloning vector

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Publication

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Publication

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF

Plasmid

#26262

Depositor

Tom Clandinin

Publication

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

Multiple cloning site

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pSD025A

Plasmid

#218128

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

Depositor

David Liu

Publication

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CRBN (CRBN Human)
M13 pIII-neg

Tags

scP22

Mutation

W384A, Y386A

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Olônne

Plasmid

#214443

Purpose

BxbI landing-pad HDR donor for targeting Olônne-18 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Publication

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDuex2-rsTagRFP

Plasmid

#205757

Purpose

Expression of rsTagRFP in mamalian cells

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rsTagRFP

Expression

Mammalian

Mutation

wt

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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