166,289 results

1. YFP NLS Beta-Actin G13R

   Plasmid

   #60615

   Purpose

   Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   G13R

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-No-HaloTag7-mGFP-LC3-mRFP

   Plasmid

   #184902

   Purpose

   Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Tags

   HaloTag7, monomeric EGFP, and monomeric RFP

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

   Plasmid

   #199359

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Promoter

   pRhaBAD

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Tags

   his

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ1996_v2

   Plasmid

   #140664

   Purpose

   dCas9 & Csy4

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   dCas9 & Csy4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

   Plasmid

   #184635

   Purpose

   Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH3.3_Cre-MS2x12

   Plasmid

   #205559

   Purpose

   Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLminP_Luc2P_RE60

    Plasmid

    #90405

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT5

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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