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pGEX Smad2 (L+MH2)

Plasmid

#15730

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 (L+MH2) (SMAD2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 187-467 (linker and MH2 regions).

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
sg2.0 (PP7) Locus#2

Plasmid

#101155

Purpose

gRNA with two PCP binding sites

Depositor

Mazhar Adli

Article

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Insert

Locus #2(tandem repeat)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.PARP1#3

Plasmid

#14546

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

PARP1 shRNA (PARP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 27, 2007

Availability

Academic Institutions and Nonprofits only
Cyan Nano-lantern(Ca2+)/pcDNA3

Plasmid

#71344

Purpose

Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

Depositor

Takeharu Nagai

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Cyan Nano-lantern(Ca2+)

Expression

Mammalian

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
Twitch-2C pcDNA3

Plasmid

#104501

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pET-17b_Unc104-KIF5B-HALO

Plasmid

#169996

Purpose

Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

Depositor

Ron Vale

Article

Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

Insert

Kinesin-like protein Unc-104 fused with KIF5B

Tags

HALO-tag, 6xHIS

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

Plasmid

#191751

Purpose

Microtubule marker together with nuclear envelope marker driven by bidirectional promoter

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Inserts

NCU04288 (NCU04288 Neurospora crassa)
bml (Bml Neurospora crassa)

Tags

Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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BAS288 C terminal mKO2 natR

Plasmid

#74083

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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