We narrowed to 15,987 results for: nes
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Plasmid#35414DepositorAvailable SinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only
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pDEST53-Arl13b20
Plasmid#40876DepositorInsertArl13b (Arl13b Mouse)
TagsGFPExpressionMammalianMutationdeleted Arl N-terminal domain; added stop codonPromoterCMVAvailable SinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRET.IL (No IRES-EGFP)
Plasmid#1832DepositorTypeEmpty backboneUseCre/Lox and RetroviralExpressionMammalianAvailable SinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry
Plasmid#191500PurposeGROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eyeDepositorInsertCaSR-EGFP-SV40
UseCreation of transgenic fish using tol2 transgenes…Promotermyo6bAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-BacMam-4x-PyOtR
Plasmid#217364PurposeExpresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmidDepositorInsert4 copies of pyrrolysyl-tRNA mutant PyOtR
UseBaculoviralExpressionInsect and MammalianPromoterU6Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHTN_HaloTag_KIF5B[1-560]
Plasmid#213403PurposeTruncated human kinesin-1 with HaloTag at the N-terminusDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-mGreenLantern-KASH
Plasmid#191094PurposeTo express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertmGreenLantern-KASH
UseAAV and Synthetic BiologyTagsmGreenLantern fused to the KASH domain of Nesprin…ExpressionMammalianMutationOptimizzation was done using the Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-BAD
Plasmid#113459PurposeProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)DepositorInsertBCL2 associated agonist of cell death (BAD Human)
TagsProteinA-mCitrineExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL-CP
Plasmid#65716PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
UseLuciferaseAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV N-term HA-FLAG puro BRD7 shRescue
Plasmid#41936DepositorAvailable SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 short UTR
Plasmid#21639DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianAvailable SinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DAG1
Plasmid#205149PurposeOverexpress DAG1DepositorInsertDystroglycan 1 (DAG1 Human)
UseLentiviralExpressionMammalianMutationCarries common missense variant c.41C>G (p.Ser…PromoterEF-1aAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLI1_pLENTI-CAG-IRES-GFP
Plasmid#176992PurposeMammalian lentiviral expression vector encoding GLI1DepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_Tau
Plasmid#159717PurposeExpresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMicrotubule-associated protein tau (MAPT Human)
TagsZZ affinity tag, ybbR tagExpressionInsectAvailable SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-ERBB2-v2
Plasmid#154895PurposeExpresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assaysDepositorAvailable SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP
Plasmid#114111Purposefusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
ExpressionMammalianPromoterCAGAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorAvailable SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only