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pHR-CMV-TetO2_HA-BirA

Plasmid

#113896

Purpose

Mammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV/TO-miR-30 L5-L2

Plasmid

#62123

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Gateway Cloning and RNAi

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9A-HA

Plasmid

#209213

Purpose

Mammalian expression of ATP9A

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9A (ATP9A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6

Plasmid

#113887

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-KPTN

Plasmid

#87041

Purpose

mammalian expression of KPTN

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

KPTN (KPTN Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4

Plasmid

#177265

Purpose

STREAMING-tag cassette

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag cassette

Use

Unspecified

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

Plasmid

#60624

Purpose

Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

WT mouse MyoD (Myod1 Mouse)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-9xGRE

Plasmid

#74736

Purpose

GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

Depositor

Luisa Dalla Valle

Article

Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

Insert

EGFP

Use

Zebrafish expression

Promoter

nine GC-responsive elements (9xGRE) from the rat …

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-KRAB_Hygro

Plasmid

#192663

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al Cell Rep. 2026 Mar 24;45(3):117006. doi:

Insert

dCas9-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, D839A, H840A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc

Plasmid

#176049

Purpose

Expresses Myc tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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4928 TRV2-TnpB-HHreRNA4PDS

Plasmid

#251815

Purpose

Editing Photene desaturase (PDS) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

ISDra2 TnpB with guide targeting NbPDS flanked by HH-HDV ribozymes

Tags

AtFDnls

Expression

Plant

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAS134

Plasmid

#211505

Purpose

AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

Venus

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDMS151A_Tet-On-3G

Plasmid

#191577

Purpose

ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Tet-On(R) 3G

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Promoter

T7

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-WDR24

Plasmid

#87050

Purpose

mammalian expression of WDR24

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

WDR24 (WDR24 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Tags

3xFLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a

Plasmid

#205308

Purpose

Production of AAV particles encoding WHaloCaMP1a from the synapsin promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a

Use

AAV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG::Cytbow

Plasmid

#158995

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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