We narrowed to 10,431 results for: plasmids 101

1. TRE-UBC-mCD55-T2A-GFP

   Plasmid

   #205450

   Purpose

   lentiviral plasmid for expression of mouse CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD55 (Cd55 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiRGLO-ATF5-201

   Plasmid

   #117340

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR ATF5-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. gh141

   Plasmid

   #106811

   Purpose

   expression of gRNA targeting OGT

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   OGT (OGT Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZNF217_pLX307

   Plasmid

   #98384

   Purpose

   Lentiviral expression of ZNF217

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   ZNF217 (ZNF217 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-human-c-MYC-ER-mouse IRES-mcherry

   Plasmid

   #73570

   Purpose

   Mammalian retroviral expression of Myc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   cMYC (MYC Human)
   

   Use

   Retroviral

   Tags

   IRES-mCherry and Mouse ER

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. AAV-Syn-DIO-TRPV1-P2A-mCherry

   Plasmid

   #200831

   Purpose

   In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiV_Neo_SIK3_CR_K95M

   Plasmid

   #108106

   Purpose

   Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   SIK3 (SIK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   change guanine 501 to cytosine (silent mutation),…

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

   Plasmid

   #155306

   Purpose

   Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA BFP
   

   Expression

   Mammalian

   Promoter

   hU6, EF1a

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniCGBE1-NG (pBM1182)

   Plasmid

   #140257

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_NG-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MCS-shCHD5 #1

    Plasmid

    #68876

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. 6xHis-MBP-BrCas12b

    Plasmid

    #182276

    Purpose

    Expresses recombinant BrCas12b

    Depositor

    Piyush Jain

    Article

    Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, T7, Thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-MCS-shCHD5 #2

    Plasmid

    #68877

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pNKX2-5-T2A-GFP-PGK-Puro

    Plasmid

    #203607

    Purpose

    Donor plasmid to create 3' knockin of NKX2-5 with T2A-GFP

    Depositor

    Matthew Birket

    Article

    Douglas et al Stem Cell Res. 2023 Dec 3;74:103262. doi

    Insert

    NKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
    

    Use

    Human targeting

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV CMV mCherry-DDX21-V5

    Plasmid

    #175163

    Purpose

    Lentiviral expression of human DDX21-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

    Plasmid

    #170177

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. rag2:MYRFL_CE_pISceI

    Plasmid

    #223045

    Purpose

    Express MYRFL gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    MYRFL
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-Syn-TRPV1-P2A-DsRed

    Plasmid

    #200830

    Purpose

    Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

    Depositor

    Hong Chen

    Article

    Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

    Insert

    TRPV1-P2A-DsRed (Trpv1 Rat)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

    Plasmid

    #183536

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    ChRmine-oScarlet-Kv2.1
    

    Use

    AAV

    Mutation

    None

    Promoter

    CaMKIIa

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. (SUMO)5-(SIM)10

    Plasmid

    #126923

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Expression

    Bacterial

    Mutation

    internal BamHI site between (SUMO)5 and (SIM)10

    Promoter

    tac

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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