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pMB1-spect-PthrC-eGFP

Plasmid

#107408

Purpose

This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pZH517

Plasmid

#102670

Purpose

Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pZH512

Plasmid

#102666

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV_mIFP IRES EGFP

Plasmid

#72443

Purpose

Expressing mIFP and EGFP in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

mIFP T2A HO1 IRES EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only
(GGS)9_(+36GFP)-Cre.ape

Plasmid

#71747

Purpose

+36-GFP-Cre fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_+36GFP-Cre

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
mIFP-CytERM-N-17

Plasmid

#56217

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CytERM

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mMaroon-Tubulin-C-18

Plasmid

#56202

Purpose

Localization: Microtubules, Excitation: 610, Emission: 652

Depositor

Michael Davidson

Insert

NM_006082.2 (TUBA1B Human)

Tags

mMaroon

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos4b-Clathrin-15

Plasmid

#57506

Purpose

Localization: Clathrin Vesicles, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Clathrin (CLTB Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Dendra2-MannII-N-10

Plasmid

#57730

Purpose

Localization: Mannosidase/Golgi, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

MannII

Tags

Dendra2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mPlum-Paxillin-22

Plasmid

#55993

Purpose

Localization: Focal Adhesions, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

Paxillin (PXN Chicken)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-H1-10

Plasmid

#57383

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H1 (H1f0 Mouse)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-ARP2-N-14

Plasmid

#57349

Purpose

Localization: Actin Related Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-CathespinB-6

Plasmid

#57354

Purpose

Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CathespinB (Ctsb Rat)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-Tubulin-6

Plasmid

#57682

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
dClover2-Tubulin-C-18

Plasmid

#56376

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mKO2-Clathrin-15

Plasmid

#57863

Purpose

Localization: Clathrin Vesicles, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
mKO-Clathrin-15

Plasmid

#57833

Purpose

Localization: Clathrin Vesicles, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Insert

Clathrin (CLTB Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only