We narrowed to 13,300 results for: cDNA

1. pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

   Plasmid

   #155007

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   A1419R/P1424Q/Y1426S; insert codes for EBA175 res…

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

   Plasmid

   #155008

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   S1421R/P1424Q/Y1426S; insert codes for EBA175 res…

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

   Plasmid

   #154977

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   P1424/Y1425I/Y1426I; insert codes for EBA175 resi…

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)

   Plasmid

   #154875

   Purpose

   Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfBAEBL (PF3D7_0506900 P. falciparum)
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   I1134V, P1135L, Y1136V, F1137V; insert codes for …

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-eGFP-FRT-T0-RNF168 delta MIU1/MIU2

   Plasmid

   #133980

   Purpose

   inducible mammalian expression vector of eGFP tagged RNF168 where both motifs interacting with ubiquitin have been deleted

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of MIU1 and MIU2

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

   Plasmid

   #127403

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon, W340A,…

   Promoter

   CMV

   Available Since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

   Plasmid

   #127407

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon, K305H,…

   Promoter

   CMV

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

   Plasmid

   #127401

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon, W340A,…

   Promoter

   CMV

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

   Plasmid

   #127327

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127326

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

    Plasmid

    #108503

    Purpose

    inducible expression of vsv-INCENP d543-746-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746 (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha helix

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

    Plasmid

    #83457

    Purpose

    Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Amino-acids 4127-4303

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

    Plasmid

    #65846

    Purpose

    Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

    Plasmid

    #65847

    Purpose

    Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

    Plasmid

    #59827

    Purpose

    Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO myc Separase delta 1278-1291

    Plasmid

    #59830

    Purpose

    Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 1278-1291

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna3 T7 Jip1b(471-707)(C478S, C667S and C699T)

    Plasmid

    #52129

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    Jnk interacting protien (Jip1b)(471-707)(M1+2+6) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-470 and changed C478S, C667…

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcdna3 T7 Jip1b(471-707)(C478S, C643S and C696T)

    Plasmid

    #52130

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    Jnk interacting protein Jip1b(471-707)(M1+3+6) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-470 and changed C478S, C643…

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcdna3 T7 Jip1b(471-707)(C478S, C631S and C699T)

    Plasmid

    #52131

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    Jnk interacting protein (Jip1b)(471-707)(M1+4+6) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-470 and changed C478S, C631…

    Promoter

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid

    #50866

    Purpose

    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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