We narrowed to 23,290 results for: CRISPR

1. pET47b-T7-enAsCas12f

   Plasmid

   #204642

   Purpose

   Expression of enAsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGE014

   Plasmid

   #108682

   Purpose

   All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMEDS

   Plasmid

   #236568

   Purpose

   Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

   Depositor

   Shannon Moonah

   Article

   Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

   Inserts

   CD/UPRT (negative selection)
   NPT II (positive selection)
   

   Tags

   3x Myc and FLAG

   Expression

   Mammalian

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGE013

   Plasmid

   #108681

   Purpose

   All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-mScarlet-EFS-Blast

   Plasmid

   #227260

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIV151

   Plasmid

   #226180

   Purpose

   Expresses Cas9 in a backbone compatible with K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Promoter

   GAP1

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

   Plasmid

   #191537

   Purpose

   Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-ddMisCas13b-SNAPf-NLS-3xFlag
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB6364

    Plasmid

    #106151

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-mScarlet-EFS-Zeo

    Plasmid

    #227261

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDIV272

    Plasmid

    #226183

    Purpose

    Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p (Komagataella phaffii)

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFC334

    Plasmid

    #87846

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    yA specific sgRNA
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    A. nidulans gpdA promoter with Hammerhead ribozym…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQAT09

    Plasmid

    #223381

    Purpose

    T-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpRY-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNHEJ-recX-sacB

    Plasmid

    #158718

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Expression

    Bacterial

    Promoter

    Pmyc

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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