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We narrowed to 20,248 results for: Sele

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Showing: 7161 - 7180 of 20248 results

  1. LentiV_Neo_SIK3_CR_K95M

    Plasmid
    #108106
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Publication
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108106-plasmid-map-sequence-id-207840

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  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86611-plasmid-map-sequence-id-168847

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  4. dmBACCS2VL-IRES-dOrai

    Plasmid
    #67629
    Purpose
    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai
    Depositor
    Takao Nakata
    Publication
    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dmBACCS2VL-IRES-dOrai
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    67629-plasmid-map-sequence-id-120691

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  10. AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1

    Plasmid
    #205446
    Purpose
    human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55
    Depositor
    Deepta Bhattacharya
    Publication
    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HSV TK-H2-Kb SCT-mCD47-mCD55
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    205446-plasmid-map-sequence-id-409030

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  12. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid
    #181833
    Purpose
    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-dPlcR (K30L,K32L)
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Lysines 30 and 32 in PH domain mutated to leucine.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181833-plasmid-map-sequence-id-364220

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  13. pcDNA3-Lyn-dInPAkt(R23/25A)

    Plasmid
    #181835
    Purpose
    Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.
    Depositor
    Jin Zhang
    Publication
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-dInPAkt
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Mutation
    Arginines 23 and 25 in PH domain mutated to alani…
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181835-plasmid-map-sequence-id-364313

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  14. dCas9-DNMT3L

    Plasmid
    #126588
    Purpose
    DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3L (DNMT3L Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-dCas9-DNMT3L-NLS
    Expression
    Mammalian
    Available since
    Dec. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    126588-plasmid-map-sequence-id-244971

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  16. pEGFP-N1 hMOG-beta1-EGFP

    Plasmid
    #160978
    Purpose
    Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells
    Depositor
    Markus Reindl
    Publication
    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160978-plasmid-map-sequence-id-339525

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  17. Epac-S-H74

    Plasmid
    #170338
    Purpose
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170338-plasmid-map-sequence-id-335510

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  18. pEF1a-Usp9x_CD-mCherry

    Plasmid
    #170380
    Purpose
    Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.
    Depositor
    Miguel Ramalho-Santos
    Publication
    Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)
    Tags
    mCherry-3xFlag
    Expression
    Mammalian
    Mutation
    Catalytic domain
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170380-plasmid-map-sequence-id-335371

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  19. Venus1-S727A-STAT3

    Plasmid
    #123174
    Purpose
    Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    S727A substitution
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123174-plasmid-map-sequence-id-238641

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  20. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Publication
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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Showing: 7161 - 7180 of 20248 results