We narrowed to 687 results for: emd

1. MSP712

   Plasmid

   #65768

   Purpose

   Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   D10A and H840A mutations in Cas9

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Duet1_6xHis-TEV-eGFP-P62

   Plasmid

   #190929

   Purpose

   Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   P62 (SQSTM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-C2m2-mKate

   Plasmid

   #208765

   Purpose

   To express C2m2-mKate in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Use

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

   Plasmid

   #216386

   Purpose

   Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Dishevelled-2 (Dvl2 Mouse)
   

   Use

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

   Plasmid

   #216387

   Purpose

   Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Dishevelled-2 (Dvl2 Mouse)
   

   Use

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSems leader-ALFA-mXFP-TpoR

   Plasmid

   #222945

   Purpose

   Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   Thrombopoietin receptor (26-635) (MPL Human)
   

   Use

   Tags

   ALFAtag, Ig k-chain leader sequence, and mXFP

   Expression

   Mammalian

   Mutation

   mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET41a-MED19

   Plasmid

   #15430

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED19 (Med19 Mouse)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

10. ZIKV_Sensor_32B_LacZ

    Plasmid

    #75007

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_32B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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